Escargot and Scratch regulate neural commitment by antagonizing Notch activity in Drosophila sensory organs

Anne Ramat, Agnès Audibert, Sophie Louvet-Vallée, Françoise Simon, Pierre Fichelson, Michel Gho (Lead / Corresponding author)

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

During Notch (N)-mediated binary cell fate decisions, cells adopt two different fates according to the levels of N pathway activation: an Noff-dependent or an Non-dependent fate. How cells maintain these N activity levels over time remains largely unknown. We address this question in the cell lineage that gives rise to the Drosophila mechanosensory organs. In this lineage a primary precursor cell undergoes a stereotyped sequence of oriented asymmetric cell divisions and transits through two neural precursor states before acquiring a neuron identity. Using a combination of genetic and cell biology strategies, we show that Escargot and Scratch, two transcription factors belonging to the Snail superfamily, maintain Noff neural commitment by directly blocking the transcription of N target genes. We propose that Snail factors act by displacing proneural transcription activators from DNA binding sites. As such, Snail factors maintain the Noff state in neural precursor cells by buffering any ectopic variation in the level of N activity. Since Escargot and Scratch orthologs are present in other precursor cells, our findings are fundamental for understanding precursor cell fate acquisition in other systems.

Original languageEnglish
Pages (from-to)3024-3034
Number of pages11
JournalDevelopment
Volume143
Issue number16
Early online date28 Jul 2016
DOIs
Publication statusPublished - 15 Aug 2016

Keywords

  • Bristle lineage
  • E(spl) genes
  • Neural cell commitment
  • Notch pathway
  • Snail factors
  • Transcription repression

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    Ramat, A., Audibert, A., Louvet-Vallée, S., Simon, F., Fichelson, P., & Gho, M. (2016). Escargot and Scratch regulate neural commitment by antagonizing Notch activity in Drosophila sensory organs. Development, 143(16), 3024-3034. https://doi.org/10.1242/dev.134387