Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells

Claire E Johnson, Brett E Crawford, Marios Stavridis, Gerdy Ten Dam, Annie L Wat, Graham Rushton, Christopher M Ward, Valerie Wilson, Toin H van Kuppevelt, Jeffrey D Esko, Austin Smith, John T Gallagher, Catherine L R Merry

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    Abstract

    Embryonic stem (ES) cells can be cultured in conditions that either maintain pluripotency or allow differentiation to the three embryonic germ layers. Heparan sulfate (HS), a highly polymorphic glycosaminoglycan, is a critical cell surface coreceptor in embryogenesis, and in this paper we describe its structural transition from an unusually low-sulfated variant in ES cells to a more highly sulfated form in fluorescence-activated cell sorting-purified neural progenitor cells. The characteristic domain structure of HS was retained during this transformation. However, qualitative variations in surface sulfation patterns between ES and differentiated cells were revealed using HS epitope-specific antibodies and the HS-binding growth factor fibroblast growth factor 2 (FGF-2). Expression profiles of the HS modification enzymes indicated that both "early" (N-sulfotransferases) and "late" (6O- and 3O-sulfotransferases) sulfotransferases contributed to the alterations in sulfation patterning. An HS-null ES line was used to demonstrate the necessity for HS in neural differentiation. HS is a coreceptor for many of the protein effectors implicated in pluripotency and differentiation (e.g., members of the FGF family, bone morphogenic proteins, and fibronectin). We suggest that the stage-specific activities of these proteins are finely regulated by dynamic changes in sulfation motifs in HS chains. Disclosure of potential conflicts of interest is found at the end of this article.
    Original languageEnglish
    Pages (from-to)1913-23
    Number of pages11
    JournalStem Cells
    Volume25
    Issue number8
    DOIs
    Publication statusPublished - Aug 2007

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    Heparitin Sulfate
    Embryonic Stem Cells
    Stem Cells
    Sulfotransferases
    enhanced green fluorescent protein
    Germ Layers
    Conflict of Interest
    Proteins
    Disclosure
    Fibroblast Growth Factor 2
    Glycosaminoglycans
    Fibronectins
    Embryonic Development
    Epitopes
    Intercellular Signaling Peptides and Proteins
    Flow Cytometry
    Bone and Bones
    Antibodies

    Cite this

    Johnson, Claire E ; Crawford, Brett E ; Stavridis, Marios ; Ten Dam, Gerdy ; Wat, Annie L ; Rushton, Graham ; Ward, Christopher M ; Wilson, Valerie ; van Kuppevelt, Toin H ; Esko, Jeffrey D ; Smith, Austin ; Gallagher, John T ; Merry, Catherine L R. / Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells. In: Stem Cells. 2007 ; Vol. 25, No. 8. pp. 1913-23.
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    abstract = "Embryonic stem (ES) cells can be cultured in conditions that either maintain pluripotency or allow differentiation to the three embryonic germ layers. Heparan sulfate (HS), a highly polymorphic glycosaminoglycan, is a critical cell surface coreceptor in embryogenesis, and in this paper we describe its structural transition from an unusually low-sulfated variant in ES cells to a more highly sulfated form in fluorescence-activated cell sorting-purified neural progenitor cells. The characteristic domain structure of HS was retained during this transformation. However, qualitative variations in surface sulfation patterns between ES and differentiated cells were revealed using HS epitope-specific antibodies and the HS-binding growth factor fibroblast growth factor 2 (FGF-2). Expression profiles of the HS modification enzymes indicated that both {"}early{"} (N-sulfotransferases) and {"}late{"} (6O- and 3O-sulfotransferases) sulfotransferases contributed to the alterations in sulfation patterning. An HS-null ES line was used to demonstrate the necessity for HS in neural differentiation. HS is a coreceptor for many of the protein effectors implicated in pluripotency and differentiation (e.g., members of the FGF family, bone morphogenic proteins, and fibronectin). We suggest that the stage-specific activities of these proteins are finely regulated by dynamic changes in sulfation motifs in HS chains. Disclosure of potential conflicts of interest is found at the end of this article.",
    author = "Johnson, {Claire E} and Crawford, {Brett E} and Marios Stavridis and {Ten Dam}, Gerdy and Wat, {Annie L} and Graham Rushton and Ward, {Christopher M} and Valerie Wilson and {van Kuppevelt}, {Toin H} and Esko, {Jeffrey D} and Austin Smith and Gallagher, {John T} and Merry, {Catherine L R}",
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    Johnson, CE, Crawford, BE, Stavridis, M, Ten Dam, G, Wat, AL, Rushton, G, Ward, CM, Wilson, V, van Kuppevelt, TH, Esko, JD, Smith, A, Gallagher, JT & Merry, CLR 2007, 'Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells', Stem Cells, vol. 25, no. 8, pp. 1913-23. https://doi.org/10.1634/stemcells.2006-0445

    Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells. / Johnson, Claire E; Crawford, Brett E; Stavridis, Marios; Ten Dam, Gerdy; Wat, Annie L; Rushton, Graham; Ward, Christopher M; Wilson, Valerie; van Kuppevelt, Toin H; Esko, Jeffrey D; Smith, Austin; Gallagher, John T; Merry, Catherine L R.

    In: Stem Cells, Vol. 25, No. 8, 08.2007, p. 1913-23.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Essential alterations of heparan sulfate during the differentiation of embryonic stem cells to Sox1-enhanced green fluorescent protein-expressing neural progenitor cells

    AU - Johnson, Claire E

    AU - Crawford, Brett E

    AU - Stavridis, Marios

    AU - Ten Dam, Gerdy

    AU - Wat, Annie L

    AU - Rushton, Graham

    AU - Ward, Christopher M

    AU - Wilson, Valerie

    AU - van Kuppevelt, Toin H

    AU - Esko, Jeffrey D

    AU - Smith, Austin

    AU - Gallagher, John T

    AU - Merry, Catherine L R

    PY - 2007/8

    Y1 - 2007/8

    N2 - Embryonic stem (ES) cells can be cultured in conditions that either maintain pluripotency or allow differentiation to the three embryonic germ layers. Heparan sulfate (HS), a highly polymorphic glycosaminoglycan, is a critical cell surface coreceptor in embryogenesis, and in this paper we describe its structural transition from an unusually low-sulfated variant in ES cells to a more highly sulfated form in fluorescence-activated cell sorting-purified neural progenitor cells. The characteristic domain structure of HS was retained during this transformation. However, qualitative variations in surface sulfation patterns between ES and differentiated cells were revealed using HS epitope-specific antibodies and the HS-binding growth factor fibroblast growth factor 2 (FGF-2). Expression profiles of the HS modification enzymes indicated that both "early" (N-sulfotransferases) and "late" (6O- and 3O-sulfotransferases) sulfotransferases contributed to the alterations in sulfation patterning. An HS-null ES line was used to demonstrate the necessity for HS in neural differentiation. HS is a coreceptor for many of the protein effectors implicated in pluripotency and differentiation (e.g., members of the FGF family, bone morphogenic proteins, and fibronectin). We suggest that the stage-specific activities of these proteins are finely regulated by dynamic changes in sulfation motifs in HS chains. Disclosure of potential conflicts of interest is found at the end of this article.

    AB - Embryonic stem (ES) cells can be cultured in conditions that either maintain pluripotency or allow differentiation to the three embryonic germ layers. Heparan sulfate (HS), a highly polymorphic glycosaminoglycan, is a critical cell surface coreceptor in embryogenesis, and in this paper we describe its structural transition from an unusually low-sulfated variant in ES cells to a more highly sulfated form in fluorescence-activated cell sorting-purified neural progenitor cells. The characteristic domain structure of HS was retained during this transformation. However, qualitative variations in surface sulfation patterns between ES and differentiated cells were revealed using HS epitope-specific antibodies and the HS-binding growth factor fibroblast growth factor 2 (FGF-2). Expression profiles of the HS modification enzymes indicated that both "early" (N-sulfotransferases) and "late" (6O- and 3O-sulfotransferases) sulfotransferases contributed to the alterations in sulfation patterning. An HS-null ES line was used to demonstrate the necessity for HS in neural differentiation. HS is a coreceptor for many of the protein effectors implicated in pluripotency and differentiation (e.g., members of the FGF family, bone morphogenic proteins, and fibronectin). We suggest that the stage-specific activities of these proteins are finely regulated by dynamic changes in sulfation motifs in HS chains. Disclosure of potential conflicts of interest is found at the end of this article.

    U2 - 10.1634/stemcells.2006-0445

    DO - 10.1634/stemcells.2006-0445

    M3 - Article

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