Esterification and absorption of cholesterol: in vitro and in vivo observations in the rat

Robert J. Williams, Alun D. McCarthy, Calum D. Sutherland

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

Activity of the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) in isolated rate enterocytes was reduced by approx. 75% following a single oral dose of Sandoz compound 58-035 (30 mg·kg-1). Despite this, the formation of [14C]cholesteryl esters from [1-14C]oleic acid remained unaffected in ACAT-inhibited cell preparations. The increase in serum cholesterol concentrations observed after overnight cholesterol/cholic acid ( 1% 05%) feeding to rats was abolished by pre-treatment with Sandoz compound 58-035 (30 mg·kg-1). These results can be reconciled with a previously proposed model for the transmembrane movement of cholesterol which implicates ACAT-independent esterification and hydrolysis as a transport mechanism for the movement of cholesterol across the enterocyte apical membrane.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1003
Issue number2
DOIs
Publication statusPublished - 8 Jun 1989

Keywords

  • (Rat)
  • Absorption
  • ACAT
  • Cholesterol
  • Cholesteryl ester

Fingerprint Dive into the research topics of 'Esterification and absorption of cholesterol: in vitro and in vivo observations in the rat'. Together they form a unique fingerprint.

Cite this