Esterification and absorption of cholesterol: in vitro and in vivo observations in the rat

Robert J. Williams, Alun D. McCarthy, Calum D. Sutherland

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Activity of the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) in isolated rate enterocytes was reduced by approx. 75% following a single oral dose of Sandoz compound 58-035 (30 mg·kg-1). Despite this, the formation of [14C]cholesteryl esters from [1-14C]oleic acid remained unaffected in ACAT-inhibited cell preparations. The increase in serum cholesterol concentrations observed after overnight cholesterol/cholic acid ( 1% 05%) feeding to rats was abolished by pre-treatment with Sandoz compound 58-035 (30 mg·kg-1). These results can be reconciled with a previously proposed model for the transmembrane movement of cholesterol which implicates ACAT-independent esterification and hydrolysis as a transport mechanism for the movement of cholesterol across the enterocyte apical membrane.

Original languageEnglish
Pages (from-to)213-216
Number of pages4
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1003
Issue number2
DOIs
Publication statusPublished - 8 Jun 1989

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Esterification
Sterol O-Acyltransferase
Rats
Cholesterol
Enterocytes
Cholic Acid
Cholesterol Esters
Oleic Acid
Hydrolysis
Membranes
SAN 58035
In Vitro Techniques
Enzymes
Serum

Keywords

  • (Rat)
  • Absorption
  • ACAT
  • Cholesterol
  • Cholesteryl ester

Cite this

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Esterification and absorption of cholesterol : in vitro and in vivo observations in the rat. / Williams, Robert J.; McCarthy, Alun D.; Sutherland, Calum D.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1003, No. 2, 08.06.1989, p. 213-216.

Research output: Contribution to journalArticle

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T2 - in vitro and in vivo observations in the rat

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AU - McCarthy, Alun D.

AU - Sutherland, Calum D.

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AB - Activity of the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) in isolated rate enterocytes was reduced by approx. 75% following a single oral dose of Sandoz compound 58-035 (30 mg·kg-1). Despite this, the formation of [14C]cholesteryl esters from [1-14C]oleic acid remained unaffected in ACAT-inhibited cell preparations. The increase in serum cholesterol concentrations observed after overnight cholesterol/cholic acid ( 1% 05%) feeding to rats was abolished by pre-treatment with Sandoz compound 58-035 (30 mg·kg-1). These results can be reconciled with a previously proposed model for the transmembrane movement of cholesterol which implicates ACAT-independent esterification and hydrolysis as a transport mechanism for the movement of cholesterol across the enterocyte apical membrane.

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