Esterification and absorption of cholesterol: in vitro and in vivo observations in the rat

Robert J. Williams; Alun D. McCarthy; Calum D. Sutherland

doi:10.1016/0005-2760(89)90259-2

Esterification and absorption of cholesterol: in vitro and in vivo observations in the rat

Robert J. Williams, Alun D. McCarthy, Calum D. Sutherland

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Activity of the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) in isolated rate enterocytes was reduced by approx. 75% following a single oral dose of Sandoz compound 58-035 (30 mg·kg^-1). Despite this, the formation of [¹⁴C]cholesteryl esters from [1-¹⁴C]oleic acid remained unaffected in ACAT-inhibited cell preparations. The increase in serum cholesterol concentrations observed after overnight cholesterol/cholic acid ( 1% 05%) feeding to rats was abolished by pre-treatment with Sandoz compound 58-035 (30 mg·kg^-1). These results can be reconciled with a previously proposed model for the transmembrane movement of cholesterol which implicates ACAT-independent esterification and hydrolysis as a transport mechanism for the movement of cholesterol across the enterocyte apical membrane.

Original language	English
Pages (from-to)	213-216
Number of pages	4
Journal	Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume	1003
Issue number	2
DOIs	https://doi.org/10.1016/0005-2760(89)90259-2
Publication status	Published - 8 Jun 1989

Keywords

(Rat)
Absorption
ACAT
Cholesterol
Cholesteryl ester

ASJC Scopus subject areas

Biophysics
Biochemistry
Endocrinology

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10.1016/0005-2760(89)90259-2

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title = "Esterification and absorption of cholesterol: in vitro and in vivo observations in the rat",

abstract = "Activity of the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) in isolated rate enterocytes was reduced by approx. 75% following a single oral dose of Sandoz compound 58-035 (30 mg·kg-1). Despite this, the formation of [14C]cholesteryl esters from [1-14C]oleic acid remained unaffected in ACAT-inhibited cell preparations. The increase in serum cholesterol concentrations observed after overnight cholesterol/cholic acid ( 1% 05%) feeding to rats was abolished by pre-treatment with Sandoz compound 58-035 (30 mg·kg-1). These results can be reconciled with a previously proposed model for the transmembrane movement of cholesterol which implicates ACAT-independent esterification and hydrolysis as a transport mechanism for the movement of cholesterol across the enterocyte apical membrane.",

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AU - Williams, Robert J.

AU - McCarthy, Alun D.

AU - Sutherland, Calum D.

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N2 - Activity of the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) in isolated rate enterocytes was reduced by approx. 75% following a single oral dose of Sandoz compound 58-035 (30 mg·kg-1). Despite this, the formation of [14C]cholesteryl esters from [1-14C]oleic acid remained unaffected in ACAT-inhibited cell preparations. The increase in serum cholesterol concentrations observed after overnight cholesterol/cholic acid ( 1% 05%) feeding to rats was abolished by pre-treatment with Sandoz compound 58-035 (30 mg·kg-1). These results can be reconciled with a previously proposed model for the transmembrane movement of cholesterol which implicates ACAT-independent esterification and hydrolysis as a transport mechanism for the movement of cholesterol across the enterocyte apical membrane.

AB - Activity of the enzyme acyl-CoA:cholesterol acyltransferase (ACAT) in isolated rate enterocytes was reduced by approx. 75% following a single oral dose of Sandoz compound 58-035 (30 mg·kg-1). Despite this, the formation of [14C]cholesteryl esters from [1-14C]oleic acid remained unaffected in ACAT-inhibited cell preparations. The increase in serum cholesterol concentrations observed after overnight cholesterol/cholic acid ( 1% 05%) feeding to rats was abolished by pre-treatment with Sandoz compound 58-035 (30 mg·kg-1). These results can be reconciled with a previously proposed model for the transmembrane movement of cholesterol which implicates ACAT-independent esterification and hydrolysis as a transport mechanism for the movement of cholesterol across the enterocyte apical membrane.

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KW - Absorption

KW - ACAT

KW - Cholesterol

KW - Cholesteryl ester

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ER -

Esterification and absorption of cholesterol: in vitro and in vivo observations in the rat

Abstract

Keywords

ASJC Scopus subject areas

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