Ethanol exposure leads to disorder of blood island formation in early chick embryo

Guang Wang, Bin-Zhen Chen, Chao-Jie Wang, Jing Zhang, Lin-rui Gao, Manli Chuai, Yongping Bao, Xuesong Yang (Lead / Corresponding author)

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Abstract

Ethanol's effect on embryonic vasculogenesis and its underlying mechanism is obscure. Using VE-cadherin in situ hybridization, we found blood islands formation was inhibited in area opaca, but abnormal VE-cadherin(+) cells were seen in area pellucida. We hypothesise ethanol may affect blood island progenitor cell migration and differentiation. DiI and in vitro experiments revealed ethanol inhibited cell migration, Quantitative PCR analysis revealed that ethanol exposure enhanced cell differentiation in area pellucida of HH5 chick embryos and repressed cell differentiation in area pellucida of HH8 chick embryos. By exposing to 2,2'-azobis-amidinopropane dihydrochloride, a ROS inducer, which gave a similar anti-vasculogenesis effect as ethanol and this anti-vasculogenesis effect could be reversed by vitamin C. Overall, exposing early chick embryos to ethanol represses blood island progenitor cell migration but disturbed differentiation at a different stage, so that the disorder of blood island formation occurs through excess ROS production and altered vascular-associated gene expression.

Original languageEnglish
Pages (from-to)96-104
Number of pages9
JournalReproductive Toxicology
Volume73
Early online date5 Aug 2017
DOIs
Publication statusPublished - Oct 2017

Keywords

  • Ethanol
  • Vasculogenesis
  • Chick embryo
  • Blood island
  • ROS

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