Eukaryotic replisome components cooperate to process histones during chromosome replication

Magdalena Foltman, Cecile Evrin, Giacomo De Piccoli, Richard C. Jones, Rick D. Edmondson, Yuki Katou, Ryuichiro Nakato, Katsuhiko Shirahige, Karim Labib (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    82 Citations (Scopus)

    Abstract

    DNA unwinding at eukaryotic replication forks displaces parental histones, which must be redeposited onto nascent DNA in order to preserve chromatin structure. By screening systematically for replisome components that pick up histones released from chromatin into a yeast cell extract, we found that the Mcm2 helicase subunit binds histones cooperatively with the FACT (facilitiates chromatin transcription) complex, which helps to re-establish chromatin during transcription. FACT does not associate with the Mcm2-7 helicase at replication origins during G1 phase but is subsequently incorporated into the replisome progression complex independently of histone binding and uniquely among histone chaperones. The amino terminal tail of Mcm2 binds histones via a conserved motif that is dispensable for DNA synthesis per se but helps preserve subtelomeric chromatin, retain the 2 micron minichromosome, and support growth in the absence of Ctf18-RFC. Our data indicate that the eukaryotic replication and transcription machineries use analogous assemblies of multiple chaperones to preserve chromatin integrity.
    Original languageEnglish
    Pages (from-to)892-904
    Number of pages13
    JournalCell Reports
    Volume3
    Issue number3
    DOIs
    Publication statusPublished - 28 Mar 2013

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  • Cite this

    Foltman, M., Evrin, C., De Piccoli, G., Jones, R. C., Edmondson, R. D., Katou, Y., Nakato, R., Shirahige, K., & Labib, K. (2013). Eukaryotic replisome components cooperate to process histones during chromosome replication. Cell Reports, 3(3), 892-904. https://doi.org/10.1016/j.celrep.2013.02.028