Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures

Mohammad Faisal J. Khan; Julian Little; Peter A. Mossey; Régine P. M. Steegers-Theunissen; Luca Autelitano; Ilenia  Lombardo; Rita Bassi  Andreasi; Michele Rubini

doi:10.2217/epi-2017-0081

Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures

Mohammad Faisal J. Khan, Julian Little, Peter A. Mossey, Régine P. M. Steegers-Theunissen, Luca Autelitano, Ilenia Lombardo, Rita Bassi Andreasi, Michele Rubini (Lead / Corresponding author)

Dentistry

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

309 Downloads (Pure)

Abstract

AIM: To pilot investigation of methylation of long interspersed nucleotide element-1 in lip tissues from infants with nonsyndromic cleft lip, and its association with maternal periconceptional exposures.

METHODS: The lateral and medial sides of the cleft lips of 23 affected infants were analyzed for long interspersed nucleotide element-1 methylation by bisulfite conversion and pyrosequencing.

RESULTS: The medial side showed 1.8% higher methylation compared with the lateral side; p = 0.031, particularly in male infants (2.7% difference; p = 0.011) or when the mothers did not take folic acid during periconceptional period (2.4% difference; p = 0.011). These results were not statistically significant when Bonferroni adjustment was used.

CONCLUSION: The observed differences in DNA methylation, although nonsignificant after correction for multiple comparisons, suggest that differential regulation of the two sides may impact lip fusion and warrant larger-scale replication.

Original language	English
Pages (from-to)	105-113
Number of pages	9
Journal	Epigenomics
Volume	10
Issue number	1
Early online date	29 Nov 2017
DOIs	https://doi.org/10.2217/epi-2017-0081
Publication status	Published - Jan 2018

Keywords

cleft lip with or without cleft palate
DNA methylation
LINE-1
Long Interspersed Nucleotide Elements/genetics
Humans
Infant
Male
Maternal Exposure
Pregnancy
DNA Methylation
Folic Acid/therapeutic use
Female
Cleft Lip/genetics
Dietary Supplements

ASJC Scopus subject areas

Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/epi-2017-0081

Author Accepted Manuscript
This is a pre-copyedited, author-version of an article accepted for publication following peer review. The version of record 'Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures', Epigenomics (2017) is available online at: https://doi.org/10.2217/epi-2017-0081.
Accepted author manuscript, 347 KB

Mossey, Peter
- Dentistry - Professor (Clinical) of Cranio Dev and Dentofacial Orthopaeds
Person: Academic

Cite this

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title = "Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures",

abstract = "AIM: To pilot investigation of methylation of long interspersed nucleotide element-1 in lip tissues from infants with nonsyndromic cleft lip, and its association with maternal periconceptional exposures.METHODS: The lateral and medial sides of the cleft lips of 23 affected infants were analyzed for long interspersed nucleotide element-1 methylation by bisulfite conversion and pyrosequencing.RESULTS: The medial side showed 1.8% higher methylation compared with the lateral side; p = 0.031, particularly in male infants (2.7% difference; p = 0.011) or when the mothers did not take folic acid during periconceptional period (2.4% difference; p = 0.011). These results were not statistically significant when Bonferroni adjustment was used.CONCLUSION: The observed differences in DNA methylation, although nonsignificant after correction for multiple comparisons, suggest that differential regulation of the two sides may impact lip fusion and warrant larger-scale replication.",

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AU - Little, Julian

AU - Mossey, Peter A.

AU - Steegers-Theunissen, Régine P. M.

AU - Autelitano, Luca

AU - Lombardo, Ilenia

AU - Andreasi, Rita Bassi

AU - Rubini, Michele

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N2 - AIM: To pilot investigation of methylation of long interspersed nucleotide element-1 in lip tissues from infants with nonsyndromic cleft lip, and its association with maternal periconceptional exposures.METHODS: The lateral and medial sides of the cleft lips of 23 affected infants were analyzed for long interspersed nucleotide element-1 methylation by bisulfite conversion and pyrosequencing.RESULTS: The medial side showed 1.8% higher methylation compared with the lateral side; p = 0.031, particularly in male infants (2.7% difference; p = 0.011) or when the mothers did not take folic acid during periconceptional period (2.4% difference; p = 0.011). These results were not statistically significant when Bonferroni adjustment was used.CONCLUSION: The observed differences in DNA methylation, although nonsignificant after correction for multiple comparisons, suggest that differential regulation of the two sides may impact lip fusion and warrant larger-scale replication.

AB - AIM: To pilot investigation of methylation of long interspersed nucleotide element-1 in lip tissues from infants with nonsyndromic cleft lip, and its association with maternal periconceptional exposures.METHODS: The lateral and medial sides of the cleft lips of 23 affected infants were analyzed for long interspersed nucleotide element-1 methylation by bisulfite conversion and pyrosequencing.RESULTS: The medial side showed 1.8% higher methylation compared with the lateral side; p = 0.031, particularly in male infants (2.7% difference; p = 0.011) or when the mothers did not take folic acid during periconceptional period (2.4% difference; p = 0.011). These results were not statistically significant when Bonferroni adjustment was used.CONCLUSION: The observed differences in DNA methylation, although nonsignificant after correction for multiple comparisons, suggest that differential regulation of the two sides may impact lip fusion and warrant larger-scale replication.

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KW - Maternal Exposure

KW - Pregnancy

KW - DNA Methylation

KW - Folic Acid/therapeutic use

KW - Female

KW - Cleft Lip/genetics

KW - Dietary Supplements

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Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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Mossey, Peter

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