Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures

TY - JOUR

T1 - Evaluating LINE-1 methylation in cleft lip tissues and its association with early pregnancy exposures

AU - Khan, Mohammad Faisal J.

AU - Little, Julian

AU - Mossey, Peter A.

AU - Steegers-Theunissen, Régine P. M.

AU - Autelitano, Luca

AU - Lombardo, Ilenia

AU - Andreasi, Rita Bassi

AU - Rubini, Michele

PY - 2018/1

Y1 - 2018/1

N2 - AIM: To pilot investigation of methylation of long interspersed nucleotide element-1 in lip tissues from infants with nonsyndromic cleft lip, and its association with maternal periconceptional exposures.METHODS: The lateral and medial sides of the cleft lips of 23 affected infants were analyzed for long interspersed nucleotide element-1 methylation by bisulfite conversion and pyrosequencing.RESULTS: The medial side showed 1.8% higher methylation compared with the lateral side; p = 0.031, particularly in male infants (2.7% difference; p = 0.011) or when the mothers did not take folic acid during periconceptional period (2.4% difference; p = 0.011). These results were not statistically significant when Bonferroni adjustment was used.CONCLUSION: The observed differences in DNA methylation, although nonsignificant after correction for multiple comparisons, suggest that differential regulation of the two sides may impact lip fusion and warrant larger-scale replication.

AB - AIM: To pilot investigation of methylation of long interspersed nucleotide element-1 in lip tissues from infants with nonsyndromic cleft lip, and its association with maternal periconceptional exposures.METHODS: The lateral and medial sides of the cleft lips of 23 affected infants were analyzed for long interspersed nucleotide element-1 methylation by bisulfite conversion and pyrosequencing.RESULTS: The medial side showed 1.8% higher methylation compared with the lateral side; p = 0.031, particularly in male infants (2.7% difference; p = 0.011) or when the mothers did not take folic acid during periconceptional period (2.4% difference; p = 0.011). These results were not statistically significant when Bonferroni adjustment was used.CONCLUSION: The observed differences in DNA methylation, although nonsignificant after correction for multiple comparisons, suggest that differential regulation of the two sides may impact lip fusion and warrant larger-scale replication.

KW - cleft lip with or without cleft palate

KW - DNA methylation

KW - LINE-1

KW - Long Interspersed Nucleotide Elements/genetics

KW - Humans

KW - Infant

KW - Male

KW - Maternal Exposure

KW - Pregnancy

KW - DNA Methylation

KW - Folic Acid/therapeutic use

KW - Female

KW - Cleft Lip/genetics

KW - Dietary Supplements

U2 - 10.2217/epi-2017-0081

DO - 10.2217/epi-2017-0081

M3 - Article

C2 - 29185345

AN - SCOPUS:85037038405

SN - 1750-1911

VL - 10

SP - 105

EP - 113

JO - Epigenomics

JF - Epigenomics

IS - 1

ER -