Evaluation of a combined laser Doppler flowmetry and iontophoresis technique for the assessment of equine cutaneous microvascular function

B. C. McGorum, A. J. Milne, W. H. Tremaine, B. P. R. Sturgeon, M. McLaren, F. Khan

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    A combined laser Doppler flowmetry and iontophoresis (LDFI) technique, used routinely to assess human microvascular function, was evaluated as a noninvasive technique for assessment of equine microvascular function, to facilitate the study of diseases such as laminitis. Baseline and vasoactive agonist-induced (acetylcholine and nitroprusside) microvascular flux was quantified at 2 sites (on the dorsal pastern adjacent to the coronary band and over the gluteals) in 6 clinically normal horses on 5 or 6 separate occasions under standardised conditions. Both agonists significantly increased microvascular flux. Skin pigmentation significantly attenuated the baseline flux, but not the magnitude of the agonist-mediated vasodilatory response. While LDFI was simple to perform, its value as a clinical and research tool for assessing the equine cutaneous microcirculation is limited by its poor reliability, as indicated by the marked intra- and intersubject variability in baseline and agonist-mediated microvascular flux.
    Original languageEnglish
    Pages (from-to)732-736
    Number of pages5
    JournalEquine Veterinary Journal
    Volume34
    Issue number7
    DOIs
    Publication statusPublished - 2002

    Fingerprint

    Iontophoresis
    Laser-Doppler Flowmetry
    agonists
    Horses
    lasers
    horses
    Skin
    Skin Pigmentation
    Nitroprusside
    Vasoconstrictor Agents
    Microcirculation
    Acetylcholine
    laminitis
    acetylcholine
    methodology
    skin (animal)
    pigmentation
    Research

    Cite this

    McGorum, B. C. ; Milne, A. J. ; Tremaine, W. H. ; Sturgeon, B. P. R. ; McLaren, M. ; Khan, F. / Evaluation of a combined laser Doppler flowmetry and iontophoresis technique for the assessment of equine cutaneous microvascular function. In: Equine Veterinary Journal. 2002 ; Vol. 34, No. 7. pp. 732-736.
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    abstract = "A combined laser Doppler flowmetry and iontophoresis (LDFI) technique, used routinely to assess human microvascular function, was evaluated as a noninvasive technique for assessment of equine microvascular function, to facilitate the study of diseases such as laminitis. Baseline and vasoactive agonist-induced (acetylcholine and nitroprusside) microvascular flux was quantified at 2 sites (on the dorsal pastern adjacent to the coronary band and over the gluteals) in 6 clinically normal horses on 5 or 6 separate occasions under standardised conditions. Both agonists significantly increased microvascular flux. Skin pigmentation significantly attenuated the baseline flux, but not the magnitude of the agonist-mediated vasodilatory response. While LDFI was simple to perform, its value as a clinical and research tool for assessing the equine cutaneous microcirculation is limited by its poor reliability, as indicated by the marked intra- and intersubject variability in baseline and agonist-mediated microvascular flux.",
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    Evaluation of a combined laser Doppler flowmetry and iontophoresis technique for the assessment of equine cutaneous microvascular function. / McGorum, B. C.; Milne, A. J.; Tremaine, W. H.; Sturgeon, B. P. R.; McLaren, M.; Khan, F.

    In: Equine Veterinary Journal, Vol. 34, No. 7, 2002, p. 732-736.

    Research output: Contribution to journalArticle

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    AU - McGorum, B. C.

    AU - Milne, A. J.

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    AU - Sturgeon, B. P. R.

    AU - McLaren, M.

    AU - Khan, F.

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    AB - A combined laser Doppler flowmetry and iontophoresis (LDFI) technique, used routinely to assess human microvascular function, was evaluated as a noninvasive technique for assessment of equine microvascular function, to facilitate the study of diseases such as laminitis. Baseline and vasoactive agonist-induced (acetylcholine and nitroprusside) microvascular flux was quantified at 2 sites (on the dorsal pastern adjacent to the coronary band and over the gluteals) in 6 clinically normal horses on 5 or 6 separate occasions under standardised conditions. Both agonists significantly increased microvascular flux. Skin pigmentation significantly attenuated the baseline flux, but not the magnitude of the agonist-mediated vasodilatory response. While LDFI was simple to perform, its value as a clinical and research tool for assessing the equine cutaneous microcirculation is limited by its poor reliability, as indicated by the marked intra- and intersubject variability in baseline and agonist-mediated microvascular flux.

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