Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection

Yosuke Obata, Gianni Ciofani, Vittoria Raffa, Alfred Cuschieri, Arianna Menciassi, Paolo Dario, Shinji Takeoka

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C(16)) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C(16) liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP- positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C(16) lipoplexes, the proportion of GFP- positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C(16), as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection.

    From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases. (C) 2010 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)70-77
    Number of pages8
    JournalNanomedicine: Nanotechnology, Biology and Medicine
    Volume6
    Issue number1
    DOIs
    Publication statusPublished - Feb 2010

    Keywords

    • Cationic liposomes
    • Amino lipids
    • Gene therapy
    • Neuronal transfection
    • Plasmid DNA
    • MEDIATED GENE-TRANSFER
    • INTERFERING RNA DELIVERY
    • PLASMID DNA DELIVERY
    • IN-VIVO
    • CELL-TRANSFECTION
    • GLIOMA-CELLS
    • VITRO
    • EXPRESSION
    • COMPLEXES
    • MECHANISM

    Cite this