Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection

Yosuke Obata, Gianni Ciofani, Vittoria Raffa, Alfred Cuschieri, Arianna Menciassi, Paolo Dario, Shinji Takeoka

    Research output: Contribution to journalArticle

    26 Citations (Scopus)

    Abstract

    We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C(16)) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C(16) liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP- positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C(16) lipoplexes, the proportion of GFP- positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C(16), as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection.

    From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases. (C) 2010 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)70-77
    Number of pages8
    JournalNanomedicine: Nanotechnology, Biology and Medicine
    Volume6
    Issue number1
    DOIs
    Publication statusPublished - Feb 2010

    Keywords

    • Cationic liposomes
    • Amino lipids
    • Gene therapy
    • Neuronal transfection
    • Plasmid DNA
    • MEDIATED GENE-TRANSFER
    • INTERFERING RNA DELIVERY
    • PLASMID DNA DELIVERY
    • IN-VIVO
    • CELL-TRANSFECTION
    • GLIOMA-CELLS
    • VITRO
    • EXPRESSION
    • COMPLEXES
    • MECHANISM

    Cite this

    Obata, Yosuke ; Ciofani, Gianni ; Raffa, Vittoria ; Cuschieri, Alfred ; Menciassi, Arianna ; Dario, Paolo ; Takeoka, Shinji. / Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection. In: Nanomedicine: Nanotechnology, Biology and Medicine. 2010 ; Vol. 6, No. 1. pp. 70-77.
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    abstract = "We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C(16)) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C(16) liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16{\%} of the cells were GFP- positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C(16) lipoplexes, the proportion of GFP- positive cells could be increased to 25{\%} by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C(16), as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection.From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases. (C) 2010 Elsevier Inc. All rights reserved.",
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    Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection. / Obata, Yosuke; Ciofani, Gianni; Raffa, Vittoria; Cuschieri, Alfred; Menciassi, Arianna; Dario, Paolo; Takeoka, Shinji.

    In: Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 6, No. 1, 02.2010, p. 70-77.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Evaluation of cationic liposomes composed of an amino acid-based lipid for neuronal transfection

    AU - Obata, Yosuke

    AU - Ciofani, Gianni

    AU - Raffa, Vittoria

    AU - Cuschieri, Alfred

    AU - Menciassi, Arianna

    AU - Dario, Paolo

    AU - Takeoka, Shinji

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    N2 - We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C(16)) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C(16) liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP- positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C(16) lipoplexes, the proportion of GFP- positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C(16), as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection.From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases. (C) 2010 Elsevier Inc. All rights reserved.

    AB - We investigated the ability of cationic liposomes composed of 1,5-dihexadecyl N-arginyl-L-glutamate (Arg-Glu2C(16)) to carry nucleic acids into neuronal cells. Such liposomes have been shown to have a remarkable capacity for transfecting immortalized cell lines. Lipoplexes between the Arg-Glu2C(16) liposomes and plasmid DNA encoding green fluorescent protein (GFP) were analyzed in terms of lipoplex formation, intracellular DNA trafficking, transfection efficiency, and cytotoxicity in neuronal SH-SY5Y cells. A maximum number of cells expressing GFP was obtained with lipoplexes at a lipid-to-DNA ratio of 15. With these lipoplexes, 16% of the cells were GFP- positive, which was approximately fourfold higher than the level obtained with a commercially available transfection reagent, Lipofectamine 2000. Furthermore, as a result of the low cytotoxicity of the Arg-Glu2C(16) lipoplexes, the proportion of GFP- positive cells could be increased to 25% by increasing the concentration of lipoplexes that was applied to the cells. We have demonstrated that Arg-Glu2C(16), as a model cationic amino acid-based lipid, has a high capability as a gene carrier, even for neuronal transfection.From the Clinical Editor: In this study, specific cationic liposomes were characterized as nucleic acid transfection agents for neuronal cells. A fourfold higher transfection rate with low cytotoxicity was reported compared to Lipofectamine 2000, a commercial reagent. The authors conclude that the studied cationic liposomes have a high capability as a gene carrier for neuronal transfection. This may become clinically significant in future gene therapy efforts of neuronal diseases. (C) 2010 Elsevier Inc. All rights reserved.

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    KW - Amino lipids

    KW - Gene therapy

    KW - Neuronal transfection

    KW - Plasmid DNA

    KW - MEDIATED GENE-TRANSFER

    KW - INTERFERING RNA DELIVERY

    KW - PLASMID DNA DELIVERY

    KW - IN-VIVO

    KW - CELL-TRANSFECTION

    KW - GLIOMA-CELLS

    KW - VITRO

    KW - EXPRESSION

    KW - COMPLEXES

    KW - MECHANISM

    U2 - 10.1016/j.nano.2009.04.005

    DO - 10.1016/j.nano.2009.04.005

    M3 - Article

    VL - 6

    SP - 70

    EP - 77

    JO - Nanomedicine: Nanotechnology, Biology and Medicine

    JF - Nanomedicine: Nanotechnology, Biology and Medicine

    SN - 1549-9634

    IS - 1

    ER -