Evaluation of human corneal ulcer healing process using optical coherence tomography: An in vitro study

Yuting Ling, Chunhui Li, Christine Purslow, Ying Yang, Zhihong Huang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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110 Downloads (Pure)

Abstract

Corneal injury is potentially leading to ulceration which remains a major health concern in ocular surface diseases. In vitro studies of new ophthalmic drugs selection are usually performed using excised cornea from slaughtered animals or laboratory animals. However, the outcomes from animal models do not completely reflect the human corneal repair and regeneration process. In vitro human corneal models suit better for the rapid testing of the drug uptake in response to drug's administration and posology. Therefore, this study aims at establishing an in-vitro 3D corneal model to characterise the corneal wound healing process. Moreover, a functional assessment of corneal morphology and strength change during the healing process is of urgent need. A phase-sensitive optical coherence tomography (OCT) system with a spectral-domain configuration was utilised to probe the structure and mechanical strength of the wounded corneal tissues. In this preliminary study, a human corneal 3D model was successfully established using tissue-engineering techniques and corneal injuries were mimicked with adjustable lesion size and depth. During the healing process, OCT provided an accurate indication of the tissue repair and regeneration. These results will be of great clinical impact to understand the biomechanics of the cornea healing process and the therapeutic effectiveness of regenerative medicine.

Original languageEnglish
Title of host publicationOptical Elastography and Tissue Biomechanics VI
EditorsGiuliano Scarcelli, Kirill V. Larin
PublisherSPIE-International Society for Optical Engineering
Number of pages7
Volume10880
ISBN (Electronic)9781510624023
DOIs
Publication statusPublished - 21 Feb 2019
EventOptical Elastography and Tissue Biomechanics VI 2019 - San Francisco, United States
Duration: 2 Feb 20193 Feb 2019

Publication series

NameOPTICAL ELASTOGRAPHY AND TISSUE BIOMECHANICS VI
ISSN (Print)0277-786X

Conference

ConferenceOptical Elastography and Tissue Biomechanics VI 2019
CountryUnited States
CitySan Francisco
Period2/02/193/02/19

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Keywords

  • Cornea
  • Human keratocytes
  • In vitro model
  • Optical coherence tomography
  • Wound healing

Cite this

Ling, Y., Li, C., Purslow, C., Yang, Y., & Huang, Z. (2019). Evaluation of human corneal ulcer healing process using optical coherence tomography: An in vitro study. In G. Scarcelli, & K. V. Larin (Eds.), Optical Elastography and Tissue Biomechanics VI (Vol. 10880). [108801H] (OPTICAL ELASTOGRAPHY AND TISSUE BIOMECHANICS VI). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2509486