Evidence for expression of heteromeric serotonin 5-HT(3) receptors in rodents

M C Hanna, P A Davies, T G Hales, E F Kirkness

    Research output: Contribution to journalArticlepeer-review

    83 Citations (Scopus)

    Abstract

    The gene and cDNAs that encode a novel subunit of rodent serotonin 5-HT(3) receptors were isolated from mouse and rat tissues. Each of the new rodent subunits shares 40% amino acid identity with the rat 5-HT(3A) subunit and 73% identity with the human 5-HT(3B) subunit. Despite a relatively low level of structural conservation, sequence analysis and functional studies suggest that the new rodent subunits are orthologues of the human 5-HT(3B) subunit. In common with homologous human receptors, rat heteromeric 5-HT(3) receptors displayed a substantially larger single-channel conductance than homomeric 5-HT(3A) receptors. In addition, the rat heteromeric receptors were less sensitive to antagonism by tubocurarine. However, in contrast to human heteromeric receptors, those of the rat displayed pronounced inward rectification of both the whole-cell and single-channel current amplitudes. Transcripts of the mouse 5-HT(3A) and 5-HT(3B) subunits are coexpressed in several cell lines that possess endogenous 5-HT(3) receptors. In addition, treatment of rat PC12 cells with nerve growth factor induced expression of both subunit mRNAs, with a similar time course for accumulation of each transcript. The combination of functional data and expression patterns is consistent with the existence of heteromeric 5-HT(3) receptors in rodent neurons.

    Original languageEnglish
    Pages (from-to)240-7
    Number of pages8
    JournalJournal of Neurochemistry
    Volume75
    Issue number1
    DOIs
    Publication statusPublished - Jul 2000

    Keywords

    • Amino Acid Sequence
    • Animals
    • Cloning, Molecular
    • DNA, Complementary
    • Electric Conductivity
    • Gene Expression
    • Humans
    • Macromolecular Substances
    • Mice
    • Molecular Sequence Data
    • PC12 Cells
    • RNA, Messenger/analysis
    • Rats
    • Receptors, Serotonin/chemistry
    • Receptors, Serotonin, 5-HT3
    • Recombinant Proteins/metabolism
    • Sequence Homology

    Fingerprint

    Dive into the research topics of 'Evidence for expression of heteromeric serotonin 5-HT(3) receptors in rodents'. Together they form a unique fingerprint.

    Cite this