Abstract
The role of guanine nucleotide binding regulatory proteins (G proteins) in the regulation of phosphorylation of the γ subunit of the CD3 antigen has been examined. CD3 γ chain phosphorylation in isolated T cell microsomes was stimulated by the G protein activator guanosine 5′-0 thiotriphosphate (GTPγS), but cyclic adenosine monophosphate and guanosine 5′-diphosphate were ineffective at inducing γ chain phosphorylation. The effect of GTPγS was rapid and transient; a half maximal effect was observed with 50μM of the nucleotide. γ polypeptide phosphorylated in vitro in GTPγS stimulated microsomes incorporated phosphate on Serines 123 and 126. These data are consistent with the involvement of a G protein in the signalling mechanisms that regulate the phosphorylation of the CD3 γ chain.
Original language | English |
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Pages (from-to) | 328-334 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 156 |
Issue number | 1 |
DOIs | |
Publication status | Published - 14 Oct 1988 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology