Evidence that a GTP binding protein regulates phosphorylation of the CD3 antigen in human T lymphocytes

TY - JOUR

T1 - Evidence that a GTP binding protein regulates phosphorylation of the CD3 antigen in human T lymphocytes

AU - Davies, Adelina A.

AU - Mark Bexham, J.

AU - Cantrell, Doreen A.

AU - Crumpton, Michael J.

PY - 1988/10/14

Y1 - 1988/10/14

N2 - The role of guanine nucleotide binding regulatory proteins (G proteins) in the regulation of phosphorylation of the γ subunit of the CD3 antigen has been examined. CD3 γ chain phosphorylation in isolated T cell microsomes was stimulated by the G protein activator guanosine 5′-0 thiotriphosphate (GTPγS), but cyclic adenosine monophosphate and guanosine 5′-diphosphate were ineffective at inducing γ chain phosphorylation. The effect of GTPγS was rapid and transient; a half maximal effect was observed with 50μM of the nucleotide. γ polypeptide phosphorylated in vitro in GTPγS stimulated microsomes incorporated phosphate on Serines 123 and 126. These data are consistent with the involvement of a G protein in the signalling mechanisms that regulate the phosphorylation of the CD3 γ chain.

AB - The role of guanine nucleotide binding regulatory proteins (G proteins) in the regulation of phosphorylation of the γ subunit of the CD3 antigen has been examined. CD3 γ chain phosphorylation in isolated T cell microsomes was stimulated by the G protein activator guanosine 5′-0 thiotriphosphate (GTPγS), but cyclic adenosine monophosphate and guanosine 5′-diphosphate were ineffective at inducing γ chain phosphorylation. The effect of GTPγS was rapid and transient; a half maximal effect was observed with 50μM of the nucleotide. γ polypeptide phosphorylated in vitro in GTPγS stimulated microsomes incorporated phosphate on Serines 123 and 126. These data are consistent with the involvement of a G protein in the signalling mechanisms that regulate the phosphorylation of the CD3 γ chain.

UR - https://www.scopus.com/pages/publications/0023766932

U2 - 10.1016/S0006-291X(88)80844-1

DO - 10.1016/S0006-291X(88)80844-1

M3 - Article

C2 - 3140812

AN - SCOPUS:0023766932

SN - 0006-291X

VL - 156

SP - 328

EP - 334

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 1

ER -