Evidence that the Ipl1-Sli15 (Aurora kinase-INCENP) complex promotes chromosome bi-orientation by altering kinetochore-spindle pole connections

Tomoyuki U. Tanaka, Najma Rachidi, Carsten Janke, Gislene Pereira, Marta Galova, Elmar Schiebel, Michael J. R. Stark, Kim Nasmyth

    Research output: Contribution to journalArticlepeer-review

    536 Citations (Scopus)

    Abstract

    How sister kinetochores attach to microtubules from opposite spindle poles during mitosis (bi-orientation) remains poorly understood. In yeast, the ortholog of the Aurora B-INCENP protein kinase complex (Ipl1-Sli15) may have a role in this crucial process, because it is necessary to prevent attachment of sister kinetochores to microtubules from the same spindle pole. We investigated IPL1 function in cells that cannot replicate their chromosomes but nevertheless duplicate their spindle pole bodies (SPBs). Kinetochores detach from old SPBs and reattach to old and new SPBs with equal frequency in IPL1+ cells, but remain attached to old SPBs in ipl1 mutants. This raises the possibility that Ipl1-Sli15 facilitates bi-orientation by promoting turnover of kinetochore-SPB connections until traction of sister kinetochores toward opposite spindle poles creates tension in the surrounding chromatin.
    Original languageEnglish
    Pages (from-to)317-329
    Number of pages13
    JournalCell
    Volume108
    Issue number3
    DOIs
    Publication statusPublished - 2002

    Keywords

    • Kinetochores
    • Microtubules
    • Mitosis

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