Ex vivo and in vivo OCT image contrast

N. Krstajić, J. Jacobs, M. Bonesi, L. E. Smith, P. Deshpande, S. MacNeil, R. Smallwood, S. J. Matcher

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

We present results of OCT and polarization-OCT applied to various ex vivo tissue samples and discuss related issues of image contrast, comparing in vivo and ex vivo preparations. Time-domain and frequency-domain OCT at 835nm and 1300nm have been applied to ex vivo skin and rabbit cornea. We can distinguish rabbit cornea epithelium for up to a month after excision. However, the skin loses all contrast upon excision and despite numerous experiments we cannot distinguish epidermis, which is clearly visible in vivo. Using a time-domain system, birefringence is clearly visible for decalcified tissue but can also be detected more weakly on fully mineralised tissue. Analysis suggests that demineralization increases the birefringence value.

Original languageEnglish
Title of host publication1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics
Volume7139
DOIs
Publication statusPublished - 30 Dec 2008
Event1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics - Canterbury, Kent, United Kingdom
Duration: 8 Sep 200810 Sep 2008

Conference

Conference1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics
CountryUnited Kingdom
CityCanterbury, Kent
Period8/09/0810/09/08

Keywords

  • Ex vivo cornea
  • Ex vivo skin
  • Optical coherence tomography
  • Tissue engineering

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  • Cite this

    Krstajić, N., Jacobs, J., Bonesi, M., Smith, L. E., Deshpande, P., MacNeil, S., Smallwood, R., & Matcher, S. J. (2008). Ex vivo and in vivo OCT image contrast. In 1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics (Vol. 7139). [71390W] https://doi.org/10.1117/12.819495