Ex vivo and in vivo OCT image contrast

N. Krstajić, J. Jacobs, M. Bonesi, L. E. Smith, P. Deshpande, S. MacNeil, R. Smallwood, S. J. Matcher

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)

    Abstract

    We present results of OCT and polarization-OCT applied to various ex vivo tissue samples and discuss related issues of image contrast, comparing in vivo and ex vivo preparations. Time-domain and frequency-domain OCT at 835nm and 1300nm have been applied to ex vivo skin and rabbit cornea. We can distinguish rabbit cornea epithelium for up to a month after excision. However, the skin loses all contrast upon excision and despite numerous experiments we cannot distinguish epidermis, which is clearly visible in vivo. Using a time-domain system, birefringence is clearly visible for decalcified tissue but can also be detected more weakly on fully mineralised tissue. Analysis suggests that demineralization increases the birefringence value.

    Original languageEnglish
    Title of host publication1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics
    Volume7139
    DOIs
    Publication statusPublished - 30 Dec 2008
    Event1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics - Canterbury, Kent, United Kingdom
    Duration: 8 Sep 200810 Sep 2008

    Conference

    Conference1st Canterbury Workshop on Optical Coherence Tomography and Adaptive Optics
    CountryUnited Kingdom
    CityCanterbury, Kent
    Period8/09/0810/09/08

    Keywords

    • Ex vivo cornea
    • Ex vivo skin
    • Optical coherence tomography
    • Tissue engineering

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