Exfoliative cytology of normal oral mucosa: Assessing the basal cell keratin phenotype

G.R. Ogden; I. Leigh; D.M. Chisholm; J.G. Cowpe; E.B. Lane

Exfoliative cytology of normal oral mucosa: Assessing the basal cell keratin phenotype

G.R. Ogden
, I. Leigh
, D.M. Chisholm
, J.G. Cowpe
, E.B. Lane

Research output: Contribution to journal › Article › peer-review

Abstract

OBJECTIVE: To attempt to evaluate the likelihood that normal keratin (antibody LH8) expression might con tribute to a false positive diagnosis when sampling a lesion affecting the oral mucosa. STUDY DESIGN: Smears were taken from four oral sites (buccal mucosa, hard palate, dorsal tongue, ventral tongue) in 28 patients using the Axibrush. These were processed using a standard avidin-biotin technique, and the number of brown 'positive' cells was assessed. RESULTS: None of the smears contained cells that were LH8 positive. CONCLUSION: While the results of this study suggest that exfoliative cytology is poor at sampling the basal cells of intact oral epithelium, it does indicate that the identification of LH8-positive cells in a smear from a clinically suspicious lesion is unlikely to be due to harvesting of normal basal cells.

Original language	English
Pages (from-to)	933-936
Number of pages	4
Journal	Acta Cytologica
Volume	40
Issue number	5
Publication status	Published - 1 Sept 1996

Cite this

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title = "Exfoliative cytology of normal oral mucosa: Assessing the basal cell keratin phenotype",

abstract = "OBJECTIVE: To attempt to evaluate the likelihood that normal keratin (antibody LH8) expression might con tribute to a false positive diagnosis when sampling a lesion affecting the oral mucosa. STUDY DESIGN: Smears were taken from four oral sites (buccal mucosa, hard palate, dorsal tongue, ventral tongue) in 28 patients using the Axibrush. These were processed using a standard avidin-biotin technique, and the number of brown 'positive' cells was assessed. RESULTS: None of the smears contained cells that were LH8 positive. CONCLUSION: While the results of this study suggest that exfoliative cytology is poor at sampling the basal cells of intact oral epithelium, it does indicate that the identification of LH8-positive cells in a smear from a clinically suspicious lesion is unlikely to be due to harvesting of normal basal cells.",

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T1 - Exfoliative cytology of normal oral mucosa

T2 - Assessing the basal cell keratin phenotype

AU - Ogden, G.R.

AU - Leigh, I.

AU - Chisholm, D.M.

AU - Cowpe, J.G.

AU - Lane, E.B.

PY - 1996/9/1

Y1 - 1996/9/1

N2 - OBJECTIVE: To attempt to evaluate the likelihood that normal keratin (antibody LH8) expression might con tribute to a false positive diagnosis when sampling a lesion affecting the oral mucosa. STUDY DESIGN: Smears were taken from four oral sites (buccal mucosa, hard palate, dorsal tongue, ventral tongue) in 28 patients using the Axibrush. These were processed using a standard avidin-biotin technique, and the number of brown 'positive' cells was assessed. RESULTS: None of the smears contained cells that were LH8 positive. CONCLUSION: While the results of this study suggest that exfoliative cytology is poor at sampling the basal cells of intact oral epithelium, it does indicate that the identification of LH8-positive cells in a smear from a clinically suspicious lesion is unlikely to be due to harvesting of normal basal cells.

AB - OBJECTIVE: To attempt to evaluate the likelihood that normal keratin (antibody LH8) expression might con tribute to a false positive diagnosis when sampling a lesion affecting the oral mucosa. STUDY DESIGN: Smears were taken from four oral sites (buccal mucosa, hard palate, dorsal tongue, ventral tongue) in 28 patients using the Axibrush. These were processed using a standard avidin-biotin technique, and the number of brown 'positive' cells was assessed. RESULTS: None of the smears contained cells that were LH8 positive. CONCLUSION: While the results of this study suggest that exfoliative cytology is poor at sampling the basal cells of intact oral epithelium, it does indicate that the identification of LH8-positive cells in a smear from a clinically suspicious lesion is unlikely to be due to harvesting of normal basal cells.

UR - https://www.scopus.com/pages/publications/0029773884

M3 - Article

AN - SCOPUS:0029773884

SN - 0001-5547

VL - 40

SP - 933

EP - 936

JO - Acta Cytologica

JF - Acta Cytologica

IS - 5

ER -

Exfoliative cytology of normal oral mucosa: Assessing the basal cell keratin phenotype

Abstract

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