Exhausted CD4+ T Cells during Malaria Exhibit Reduced mTORc1 Activity Correlated with Loss of T-bet Expression

Ana Villegas-Mendez, Garima Khandelwal, Lucy M. McGowan, Rebecca S. Dookie, Michael J. Haley, Charlotte George, David Sims, Graham M. Lord, Linda V. Sinclair, Richard G. Jenner, Kevin N. Couper

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
60 Downloads (Pure)


CD4 + T cell functional inhibition (exhaustion) is a hallmark of malaria and correlates with impaired parasite control and infection chronicity. However, the mechanisms of CD4 + T cell exhaustion are still poorly understood. In this study, we show that Ag-experienced (Ag-exp) CD4 + T cell exhaustion during Plasmodium yoelii nonlethal infection occurs alongside the reduction in mammalian target of rapamycin (mTOR) activity and restriction in CD4 + T cell glycolytic capacity. We demonstrate that the loss of glycolytic metabolism and mTOR activity within the exhausted Ag-expCD4 + T cell population during infection coincides with reduction in T-bet expression. T-bet was found to directly bind to and control the transcription of various mTOR and metabolism-related genes within effector CD4 + T cells. Consistent with this, Ag-expTh1 cells exhibited significantly higher and sustained mTOR activity than effector T-bet– (non-Th1) Ag-expT cells throughout the course of malaria. We identified mTOR to be redundant for sustaining T-bet expression in activated Th1 cells, whereas mTOR was necessary but not sufficient for maintaining IFN-g production by Th1 cells. Immunotherapy targeting PD-1, CTLA-4, and IL-27 blocked CD4 + T cell exhaustion during malaria infection and was associated with elevated T-bet expression and a concomitant increased CD4 + T cell glycolytic metabolism. Collectively, our data suggest that mTOR activity is linked to T-bet in Ag-expCD4 + T cells but that reduction in mTOR activity may not directly underpin Ag-expTh1 cell loss and exhaustion during malaria infection. These data have implications for therapeutic reactivation of exhausted CD4 + T cells during malaria infection and other chronic conditions. The Journal of Immunology, 2020, 205: 1608–1619.

Original languageEnglish
Pages (from-to)1608-1619
Number of pages12
JournalJournal of Immunology
Issue number6
Early online date17 Aug 2020
Publication statusPublished - 15 Sept 2020

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology


Dive into the research topics of 'Exhausted CD4+ T Cells during Malaria Exhibit Reduced mTORc1 Activity Correlated with Loss of T-bet Expression'. Together they form a unique fingerprint.

Cite this