Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing

Hanna Dellago, Marlies Löscher , Paul Ajuh, Ursula Ryder, Christian Kaisermayer, Regina Grillari-Voglauer, Klaus Fortschegger, Stefan Gross, Anna Gstraunthaler, Nicole Borth, Frank Eisenhaber, Angus I. Lamond, Johannes Grillari

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    20 Citations (Scopus)

    Abstract

    The Cdc5L (cell division cycle 5-like) complex is a spliceosomal subcomplex that also plays a role in DNA repair. The complex contains the splicing factor hPrp19, also known as SNEV or hPso4, which is involved in cellular life-span regulation and proteasomal breakdown. In a recent large-scale proteomics analysis for proteins associated with this complex, proteins involved in transcription, cell-cycle regulation, DNA repair, the ubiquitin-proteasome system, chromatin remodelling, cellular aging, the cytoskeleton and trafficking, including four members of the exocyst complex, were identified. In the present paper we report that Exo70 interacts directly with SNEVhPrp19/hPso4 and shuttles to the nucleus, where it associates with the spliceosome. We mapped the interaction site to the N-terminal 100 amino acids of Exo70, which interfere with pre-mRNA splicing in vitro. Furthermore, Exo70 influences the splicing of a model substrate as well as of its own pre-mRNA in vivo. In addition, we found that Exo70 is alternatively spliced in a cell-type- and cell-age-dependent way. These results suggest a novel and unexpected role of Exo70 in nuclear mRNA splicing, where it might signal membrane events to the splicing apparatus.

    Original languageEnglish
    Pages (from-to)81-91
    Number of pages11
    JournalBiochemical Journal
    Volume438
    DOIs
    Publication statusPublished - 15 Aug 2011

    Keywords

    • alternative splicing
    • exocyst
    • Exo70
    • protein interaction
    • Prp19
    • SNEV
    • NUCLEAR EXPORT SIGNALS
    • DIPLOID CELL STRAINS
    • ENDOTHELIAL-CELLS
    • MASS-SPECTROMETRY
    • SUBCELLULAR-LOCALIZATION
    • SACCHAROMYCES-CEREVISIAE
    • ENDOPLASMIC-RETICULUM
    • PROTEIN COMPLEXES
    • EPITHELIAL-CELLS
    • SEC6/8 COMPLEX

    Cite this

    Dellago, H., Löscher , M., Ajuh, P., Ryder, U., Kaisermayer, C., Grillari-Voglauer, R., Fortschegger, K., Gross, S., Gstraunthaler, A., Borth, N., Eisenhaber, F., Lamond, A. I., & Grillari, J. (2011). Exo70, a subunit of the exocyst complex, interacts with SNEVhPrp19/hPso4 and is involved in pre-mRNA splicing. Biochemical Journal, 438, 81-91. https://doi.org/10.1042/BJ20110183