Projects per year
Exome capture is a reduced representation approach that selectively captures sequence from only the gene-bearing regions of a genome. It is based on probes targeted at these regions and, compared with whole genome shotgun sequencing, leads to a significant reduction in cost and data processing effort while still providing insights into the most relevant part of a genome. An exome capture array for barley was released in 2013 and this has opened the door to numerous studies that have put this technology to good use. In this chapter we detail the laboratory protocols required for enrichment and sequencing, and provide detailed step-by-step instructions for the bioinformatics analysis of the resulting data.
|Title of host publication||Barley|
|Subtitle of host publication||Methods and Protocols|
|Editors||Wendy A. Harwood|
|Place of Publication||New York|
|Number of pages||28|
|Publication status||Published - 2019|
|Name||Methods in Molecular Biology|
- Barley exome capture
- Data analysis
- Library preparation
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- 1 Finished
An Integrated Approach to Evaluate and Harvest Genetic Diversity for Breeding Climate-Resistent Barley (FACCE-JPI ERANET) (Joint with Univerities of Helsinki, Copenhagen, East Anglia, Milan, Hebrew University of Jerusalem, Spanish National Research Council, Leibniz Institute of Plant Genetic and Crop Pland Research and Agricultural Research Council, Spain)
2/02/15 → 1/02/18