Exome Capture for Variant Discovery and Analysis in Barley

Micha Bayer; Jenny A. Morris; Clare Booth; Allan Booth; Niki Uzrek; Joanne R. Russell; Robbie Waugh; Pete E. Hedley

doi:10.1007/978-1-4939-8944-7_18

Exome Capture for Variant Discovery and Analysis in Barley

Micha Bayer (Lead / Corresponding author), Jenny A. Morris, Clare Booth, Allan Booth, Niki Uzrek, Joanne R. Russell, Robbie Waugh, Pete E. Hedley

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

7 Citations (Scopus)

330 Downloads (Pure)

Abstract

Exome capture is a reduced representation approach that selectively captures sequence from only the gene-bearing regions of a genome. It is based on probes targeted at these regions and, compared with whole genome shotgun sequencing, leads to a significant reduction in cost and data processing effort while still providing insights into the most relevant part of a genome. An exome capture array for barley was released in 2013 and this has opened the door to numerous studies that have put this technology to good use. In this chapter we detail the laboratory protocols required for enrichment and sequencing, and provide detailed step-by-step instructions for the bioinformatics analysis of the resulting data.

Original language	English
Title of host publication	Barley
Subtitle of host publication	Methods and Protocols
Editors	Wendy A. Harwood
Place of Publication	New York
Publisher	Humana Press
Pages	283-310
Number of pages	28
Volume	1900
ISBN (Electronic)	9781493989447
ISBN (Print)	9781493989423
DOIs	https://doi.org/10.1007/978-1-4939-8944-7_18
Publication status	Published - 2019

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
ISSN (Print)	1064-3745

Keywords

Barley exome capture
Bioinformatics
Data analysis
Enrichment
Library preparation

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-4939-8944-7_18

Author Accepted ManuscriptAccepted author manuscript, 1.18 MB

Cite this

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Exome Capture for Variant Discovery and Analysis in Barley. / Bayer, Micha (Lead / Corresponding author); Morris, Jenny A.; Booth, Clare et al.
Barley: Methods and Protocols. ed. / Wendy A. Harwood. Vol. 1900 New York: Humana Press, 2019. p. 283-310 (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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AU - Bayer, Micha

AU - Morris, Jenny A.

AU - Booth, Clare

AU - Booth, Allan

AU - Uzrek, Niki

AU - Russell, Joanne R.

AU - Waugh, Robbie

AU - Hedley, Pete E.

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AB - Exome capture is a reduced representation approach that selectively captures sequence from only the gene-bearing regions of a genome. It is based on probes targeted at these regions and, compared with whole genome shotgun sequencing, leads to a significant reduction in cost and data processing effort while still providing insights into the most relevant part of a genome. An exome capture array for barley was released in 2013 and this has opened the door to numerous studies that have put this technology to good use. In this chapter we detail the laboratory protocols required for enrichment and sequencing, and provide detailed step-by-step instructions for the bioinformatics analysis of the resulting data.

KW - Barley exome capture

KW - Bioinformatics

KW - Data analysis

KW - Enrichment

KW - Library preparation

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CY - New York

ER -

Exome Capture for Variant Discovery and Analysis in Barley

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Exome Capture for Variant Discovery and Analysis in Barley

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