Exploring the genetic architecture of inflammatory bowel disease by whole-genome sequencing identifies association at ADCY7

Yang Luo, Katrina M. de Lange, Luke Jostins, Loukas Moutsianas, Joshua Randall, Nicholas A. Kennedy, Christopher A. Lamb, Shane McCarthy, Tariq Ahmad, Cathryn Edwards, Eva Goncalves Serra, Ailsa Hart, Chris Hawkey, John C. Mansfield, Craig Mowat, William G. Newman, Sam Nichols, Martin Pollard, Jack Satsangi, Alison SimmonsMark Tremelling, Holm Uhlig, David C. Wilson, James C. Lee, Natalie J. Prescott, Charlie W. Lees, Christopher G. Mathew, Miles Parkes, Jeffrey C. Barrett, Carl A. Anderson

    Research output: Contribution to journalArticle

    54 Citations (Scopus)

    Abstract

    To further resolve the genetic architecture of the inflammatory bowel diseases ulcerative colitis and Crohn's disease, we sequenced the whole genomes of 4,280 patients at low coverage and compared them to 3,652 previously sequenced population controls across 73.5 million variants. We then imputed from these sequences into new and existing genome-wide association study cohorts and tested for association at ∼12 million variants in a total of 16,432 cases and 18,843 controls. We discovered a 0.6% frequency missense variant in ADCY7 that doubles the risk of ulcerative colitis. Despite good statistical power, we did not identify any other new low-frequency risk variants and found that such variants explained little heritability. We detected a burden of very rare, damaging missense variants in known Crohn's disease risk genes, suggesting that more comprehensive sequencing studies will continue to improve understanding of the biology of complex diseases.

    Original languageEnglish
    Pages (from-to)186-192
    Number of pages9
    JournalNature Genetics
    Volume49
    Issue number2
    DOIs
    Publication statusPublished - 9 Jan 2017

    Keywords

    • DNA sequencing
    • Genetic association study
    • Inflammatory bowel disease

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    Cite this

    Luo, Y., de Lange, K. M., Jostins, L., Moutsianas, L., Randall, J., Kennedy, N. A., Lamb, C. A., McCarthy, S., Ahmad, T., Edwards, C., Serra, E. G., Hart, A., Hawkey, C., Mansfield, J. C., Mowat, C., Newman, W. G., Nichols, S., Pollard, M., Satsangi, J., ... Anderson, C. A. (2017). Exploring the genetic architecture of inflammatory bowel disease by whole-genome sequencing identifies association at ADCY7. Nature Genetics, 49(2), 186-192. https://doi.org/10.1038/ng.3761