The elucidation of protein-protein interaction networks can provide preliminary insights into the function of uncharacterized proteins based on the interactions they establish in the cell. Here, we describe a protein immunoprecipitation protocol that can be used in combination with mass spectrometry analysis to identify the p97 interactome as well as specific subgroups of proteins interacting with its UBX-domain adaptors. This approach aims to dissect the role played by individual UBX cofactors within the complex array of cellular functions performed by p97.
|Title of host publication||Ubiquitin family modifiers and the proteasome|
|Subtitle of host publication||reviews and protocols|
|Editors||R. Jürgen Dohmen , Martin Scheffner|
|Number of pages||8|
|Publication status||Published - 2012|
|Name||Methods in molecular biology|
Alexandru, G. (2012). Exploring the role of p97 and its UBX-domain cofactors through identification of their interacting proteins. In R. J. Dohmen , & M. Scheffner (Eds.), Ubiquitin family modifiers and the proteasome: reviews and protocols (pp. 305-312). (Methods in molecular biology; Vol. 832). Humana Press. https://doi.org/10.1007/978-1-61779-474-2_21