Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes

Clare Stretton, Gary J. Litherland, Audrey Moynihan, Eric Hajduch, Harinder S. Hundal

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    tub encodes a protein of poorly understood function, but one implicated strongly in the control of energy balance and insulin sensitivity. Whilst tub expression is particularly prominent in neurones it is also detectable in extraneuronal tissues. We show here, for the first time, expression of TUB protein in rat adipocytes and the murine adipocyte model 3T3-L1 and demonstrate that insulin induces its tyrosine phosphorylation and association with the insulin receptor. TUB expression is regulated developmentally during adipogenic differentiation of 3T3-L1 cells and in response to cell treatment with thyroid hormone or induction of insulin resistance. TUB was upregulated 5- to 10-fold in adipocytes from obese Zucker rats and 3T3-L1 adipocytes that had been rendered insulin resistant, a response that could be antagonised by rosiglitasone, an insulin-sensitising drug. Our data are consistent with a previously unforeseen role for TUB in insulin signalling and fuel homeostasis in adipocytes. (C) 2009 Elsevier Inc. All rights reserved.

    Original languageEnglish
    Pages (from-to)1328-1333
    Number of pages6
    JournalBiochemical and Biophysical Research Communications
    Volume390
    Issue number4
    DOIs
    Publication statusPublished - 25 Dec 2009

    Keywords

    • 3T3-L1
    • Adipogenesis
    • Zucker rat
    • Insulin
    • Tubby
    • CAENORHABDITIS-ELEGANS
    • GENE FAMILY
    • MOLECULAR CHARACTERIZATION
    • C-ELEGANS
    • LIFE-SPAN
    • PROTEINS
    • MOUSE
    • OBESITY
    • FAT
    • TRANSPORT

    Cite this

    Stretton, Clare ; Litherland, Gary J. ; Moynihan, Audrey ; Hajduch, Eric ; Hundal, Harinder S. / Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 390, No. 4. pp. 1328-1333.
    @article{764953b784a94324bd2875f113025b15,
    title = "Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes",
    abstract = "tub encodes a protein of poorly understood function, but one implicated strongly in the control of energy balance and insulin sensitivity. Whilst tub expression is particularly prominent in neurones it is also detectable in extraneuronal tissues. We show here, for the first time, expression of TUB protein in rat adipocytes and the murine adipocyte model 3T3-L1 and demonstrate that insulin induces its tyrosine phosphorylation and association with the insulin receptor. TUB expression is regulated developmentally during adipogenic differentiation of 3T3-L1 cells and in response to cell treatment with thyroid hormone or induction of insulin resistance. TUB was upregulated 5- to 10-fold in adipocytes from obese Zucker rats and 3T3-L1 adipocytes that had been rendered insulin resistant, a response that could be antagonised by rosiglitasone, an insulin-sensitising drug. Our data are consistent with a previously unforeseen role for TUB in insulin signalling and fuel homeostasis in adipocytes. (C) 2009 Elsevier Inc. All rights reserved.",
    keywords = "3T3-L1, Adipogenesis, Zucker rat, Insulin, Tubby, CAENORHABDITIS-ELEGANS, GENE FAMILY, MOLECULAR CHARACTERIZATION, C-ELEGANS, LIFE-SPAN, PROTEINS, MOUSE, OBESITY, FAT, TRANSPORT",
    author = "Clare Stretton and Litherland, {Gary J.} and Audrey Moynihan and Eric Hajduch and Hundal, {Harinder S.}",
    year = "2009",
    month = "12",
    day = "25",
    doi = "10.1016/j.bbrc.2009.10.147",
    language = "English",
    volume = "390",
    pages = "1328--1333",
    journal = "Biochemical and Biophysical Research Communications",
    issn = "0006-291X",
    publisher = "Elsevier",
    number = "4",

    }

    Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes. / Stretton, Clare; Litherland, Gary J.; Moynihan, Audrey; Hajduch, Eric; Hundal, Harinder S.

    In: Biochemical and Biophysical Research Communications, Vol. 390, No. 4, 25.12.2009, p. 1328-1333.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Expression and modulation of TUB by insulin and thyroid hormone in primary rat and murine 3T3-L1 adipocytes

    AU - Stretton, Clare

    AU - Litherland, Gary J.

    AU - Moynihan, Audrey

    AU - Hajduch, Eric

    AU - Hundal, Harinder S.

    PY - 2009/12/25

    Y1 - 2009/12/25

    N2 - tub encodes a protein of poorly understood function, but one implicated strongly in the control of energy balance and insulin sensitivity. Whilst tub expression is particularly prominent in neurones it is also detectable in extraneuronal tissues. We show here, for the first time, expression of TUB protein in rat adipocytes and the murine adipocyte model 3T3-L1 and demonstrate that insulin induces its tyrosine phosphorylation and association with the insulin receptor. TUB expression is regulated developmentally during adipogenic differentiation of 3T3-L1 cells and in response to cell treatment with thyroid hormone or induction of insulin resistance. TUB was upregulated 5- to 10-fold in adipocytes from obese Zucker rats and 3T3-L1 adipocytes that had been rendered insulin resistant, a response that could be antagonised by rosiglitasone, an insulin-sensitising drug. Our data are consistent with a previously unforeseen role for TUB in insulin signalling and fuel homeostasis in adipocytes. (C) 2009 Elsevier Inc. All rights reserved.

    AB - tub encodes a protein of poorly understood function, but one implicated strongly in the control of energy balance and insulin sensitivity. Whilst tub expression is particularly prominent in neurones it is also detectable in extraneuronal tissues. We show here, for the first time, expression of TUB protein in rat adipocytes and the murine adipocyte model 3T3-L1 and demonstrate that insulin induces its tyrosine phosphorylation and association with the insulin receptor. TUB expression is regulated developmentally during adipogenic differentiation of 3T3-L1 cells and in response to cell treatment with thyroid hormone or induction of insulin resistance. TUB was upregulated 5- to 10-fold in adipocytes from obese Zucker rats and 3T3-L1 adipocytes that had been rendered insulin resistant, a response that could be antagonised by rosiglitasone, an insulin-sensitising drug. Our data are consistent with a previously unforeseen role for TUB in insulin signalling and fuel homeostasis in adipocytes. (C) 2009 Elsevier Inc. All rights reserved.

    KW - 3T3-L1

    KW - Adipogenesis

    KW - Zucker rat

    KW - Insulin

    KW - Tubby

    KW - CAENORHABDITIS-ELEGANS

    KW - GENE FAMILY

    KW - MOLECULAR CHARACTERIZATION

    KW - C-ELEGANS

    KW - LIFE-SPAN

    KW - PROTEINS

    KW - MOUSE

    KW - OBESITY

    KW - FAT

    KW - TRANSPORT

    U2 - 10.1016/j.bbrc.2009.10.147

    DO - 10.1016/j.bbrc.2009.10.147

    M3 - Article

    C2 - 19887065

    VL - 390

    SP - 1328

    EP - 1333

    JO - Biochemical and Biophysical Research Communications

    JF - Biochemical and Biophysical Research Communications

    SN - 0006-291X

    IS - 4

    ER -