Expression and regulation of prostaglandin E receptor subtype mRNAs in rat sensory ganglia and spinal cord in response to peripheral inflammation

Lucy F. Donaldson, Peter S. Humphrey, Sue Oldfield, Susan Giblett, Blair D. Grubb

    Research output: Contribution to journalArticle

    40 Citations (Scopus)

    Abstract

    Prostaglandins are known to act via seven transmembrane domain receptors to exert actions on both peripheral and central neurons resulting in changes in neuronal excitability. Prostaglandin E2, the prostaglandin most often associated with inflammation, itself acts on a family of closely related receptors, the EP receptors. Using semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), we have shown that rat primary afferent neurons express the mRNA for all EP receptor subtypes, and that some, but not all EP receptor subtype mRNAs are down-regulated in sensory neurons in response to an acute peripheral inflammation. We also show for the first time that all EP receptor subtype mRNAs are expressed in rat lumbar spinal cord. Spinal cord EP receptor subtype mRNAs are also regulated in acute inflammation in a pattern distinct from the changes seen in sensory ganglia in response to the same inflammatory stimulus.

    Original languageEnglish
    Pages (from-to)109-122
    Number of pages14
    JournalProstaglandins and Other Lipid Mediators
    Volume63
    Issue number3
    DOIs
    Publication statusPublished - Jan 2001

    Keywords

    • Adjuvant inflammation
    • mRNA Expression
    • Prostanoid receptor
    • Rat

    Fingerprint Dive into the research topics of 'Expression and regulation of prostaglandin E receptor subtype mRNAs in rat sensory ganglia and spinal cord in response to peripheral inflammation'. Together they form a unique fingerprint.

  • Cite this