Expression of an abundant alpha-class glutathione S-transferase in bovine and human adrenal cortex tissues

I Meikle, J D Hayes, S W Walker

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    Bovine adrenal cortex tissue expresses high levels of glutathione S-transferase (GST) from each of the alpha, mu and pi gene families. We describe the purification and characterization of an abundant alpha-class GST from this tissue that has not been identified previously because of its failure to bind to S-hexylglutathione-Sepharose 6B (S-hexG-Ag). This enzyme has been affinity purified on glutathione-Sepharose 6B (GSH-Ag) and was obtained in a highly purified form by employing S-hexG-Ag to remove the bulk of GST before chromatography on GSH-Ag. The purified GST eluted from GSH-Ag was found to exhibit marked peroxidase and delta 5-ketosteroid isomerase activities (19.2 and 1.67 U/mg respectively). The bovine enzyme also showed high GST activity towards 4-hydroxynonenal (5.09 U/mg). Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed that the bovine GST contains two distinct polypeptides, one with an Mr of 25,900 and the other with an Mr of 26,500. An abundant alpha-class GST was also purified from human adrenal cortex that possessed properties which were similar to the bovine alpha-class GST described above; however, unlike the bovine enzyme, the corresponding human alpha-class GST bound to S-hexG-Ag. As with the bovine enzyme, the purified human GST displayed marked peroxidase and isomerase activities (27 and 4.02 U/mg respectively). Further analysis on SDS-PAGE (Mr 25,800) and reverse-phase high-performance liquid chromatography established that this abundant alpha-class GST in human adrenal cortex is equivalent to the human liver GST B1B1 enzyme. As both human and bovine adrenal cortex contain high levels of alpha-class GST with similar catalytic properties, we discuss the possible functions of these enzymes in this tissue.

    Original languageEnglish
    Pages (from-to)83-92
    Number of pages10
    JournalJournal of Endocrinology
    Volume132
    Issue number1
    DOIs
    Publication statusPublished - Jan 1992

    Keywords

    • Adrenal Cortex/enzymology
    • Animals
    • Cattle
    • Chromatography, Gel
    • Chromatography, High Pressure Liquid
    • Cytosol/enzymology
    • Electrophoresis, Polyacrylamide Gel
    • Glutathione Transferase/analysis
    • Humans
    • Liver/enzymology

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