TY - JOUR
T1 - Expression of the murine glutathione S-transferase α3 (GSTA3) subunit is markedly induced during adipocyte differentiation
T2 - activation of the GSTA3 gene promoter by the pro-adipogenic eicosanoid 15-deoxy-Δ12,14-prostaglandin J2
AU - Jowsey, Ian R.
AU - Smith, Stephen A.
AU - Hayes, John D.
PY - 2003
Y1 - 2003
N2 - The expression of class alpha, micro, and pi glutathione S-transferases (GSTs) has been examined during the adipose conversion of mouse 3T3-L1 cells. The GSTA4, GSTM1, and GSTP1/2 subunits are expressed constitutively in confluent 3T3-L1 cells, and their levels remain essentially unchanged during adipocyte differentiation. By contrast, the GSTA3 subunit is virtually undetectable in confluent 3T3-L1 cells under basal conditions, but its expression is markedly induced during adipose conversion. Inhibition of the 3T3-L1 adipogenic program demonstrated that GSTA3 expression is associated specifically with acquisition of the adipocytic phenotype. Reporter gene assays demonstrated that the mouse GSTA3 5(')-upstream region is transcriptionally activated by 15-deoxy-Delta(12,14)-prostaglandin J(2) through an antioxidant response element, suggesting that this pro-adipogenic eicosanoid may be involved in regulating GSTA3 expression during adipogenesis. These data suggest a previously unrecognised role for GSTs in mouse adipocytes.
AB - The expression of class alpha, micro, and pi glutathione S-transferases (GSTs) has been examined during the adipose conversion of mouse 3T3-L1 cells. The GSTA4, GSTM1, and GSTP1/2 subunits are expressed constitutively in confluent 3T3-L1 cells, and their levels remain essentially unchanged during adipocyte differentiation. By contrast, the GSTA3 subunit is virtually undetectable in confluent 3T3-L1 cells under basal conditions, but its expression is markedly induced during adipose conversion. Inhibition of the 3T3-L1 adipogenic program demonstrated that GSTA3 expression is associated specifically with acquisition of the adipocytic phenotype. Reporter gene assays demonstrated that the mouse GSTA3 5(')-upstream region is transcriptionally activated by 15-deoxy-Delta(12,14)-prostaglandin J(2) through an antioxidant response element, suggesting that this pro-adipogenic eicosanoid may be involved in regulating GSTA3 expression during adipogenesis. These data suggest a previously unrecognised role for GSTs in mouse adipocytes.
U2 - 10.1016/j.bbrc.2003.11.068
DO - 10.1016/j.bbrc.2003.11.068
M3 - Article
C2 - 14652005
SN - 0006-291X
VL - 312
SP - 1226
EP - 1235
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -