Expression of transcription factor AML-2 (RUNX3, CBF(alpha)-3) is induced by Epstein-Barr virus EBNA-2 and correlates with the B-cell activation phenotype

Lindsay C Spender, Georgina H Cornish, Alexandra Sullivan, Paul J Farrell (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    60 Citations (Scopus)

    Abstract

    To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.

    Original languageEnglish
    Pages (from-to)4919-4927
    Number of pages9
    JournalJournal of Virology
    Volume76
    Issue number10
    DOIs
    Publication statusPublished - May 2002

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    Keywords

    • Adaptor Proteins, Signal Transducing
    • B-Lymphocytes
    • Burkitt Lymphoma
    • Carrier Proteins
    • Cell Line
    • Cell Transformation, Viral
    • Cells, Cultured
    • Core Binding Factor Alpha 2 Subunit
    • Core Binding Factor Alpha 3 Subunit
    • Cytoskeletal Proteins
    • DNA-Binding Proteins
    • Epstein-Barr Virus Nuclear Antigens
    • Gene Expression Profiling
    • Herpesvirus 4, Human
    • Humans
    • Interleukin-16
    • Intracellular Signaling Peptides and Proteins
    • LIM Domain Proteins
    • Lymphocyte Activation
    • Oligonucleotide Array Sequence Analysis
    • Phenotype
    • Proto-Oncogene Proteins
    • Transcription Factors
    • Tumor Cells, Cultured
    • Virus Latency
    • Comparative Study
    • Journal Article

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