Abstract
To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.
Original language | English |
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Pages (from-to) | 4919-4927 |
Number of pages | 9 |
Journal | Journal of Virology |
Volume | 76 |
Issue number | 10 |
DOIs | |
Publication status | Published - May 2002 |
Keywords
- Adaptor Proteins, Signal Transducing
- B-Lymphocytes
- Burkitt Lymphoma
- Carrier Proteins
- Cell Line
- Cell Transformation, Viral
- Cells, Cultured
- Core Binding Factor Alpha 2 Subunit
- Core Binding Factor Alpha 3 Subunit
- Cytoskeletal Proteins
- DNA-Binding Proteins
- Epstein-Barr Virus Nuclear Antigens
- Gene Expression Profiling
- Herpesvirus 4, Human
- Humans
- Interleukin-16
- Intracellular Signaling Peptides and Proteins
- LIM Domain Proteins
- Lymphocyte Activation
- Oligonucleotide Array Sequence Analysis
- Phenotype
- Proto-Oncogene Proteins
- Transcription Factors
- Tumor Cells, Cultured
- Virus Latency
- Comparative Study
- Journal Article