Expression of transcription factor AML-2 (RUNX3, CBF(alpha)-3) is induced by Epstein-Barr virus EBNA-2 and correlates with the B-cell activation phenotype

Lindsay C Spender, Georgina H Cornish, Alexandra Sullivan, Paul J Farrell (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    59 Citations (Scopus)

    Abstract

    To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.

    Original languageEnglish
    Pages (from-to)4919-4927
    Number of pages9
    JournalJournal of Virology
    Volume76
    Issue number10
    DOIs
    Publication statusPublished - May 2002

    Fingerprint

    Human herpesvirus 4
    Human Herpesvirus 4
    B-lymphocytes
    B-Lymphocytes
    Transcription Factors
    transcription factors
    Virus Latency
    Phenotype
    phenotype
    Interleukin-16
    cell lines
    Cell Line
    Virus Diseases
    interleukins
    cells
    Genes
    Proteins
    cytokines
    genes
    proteins

    Keywords

    • Adaptor Proteins, Signal Transducing
    • B-Lymphocytes
    • Burkitt Lymphoma
    • Carrier Proteins
    • Cell Line
    • Cell Transformation, Viral
    • Cells, Cultured
    • Core Binding Factor Alpha 2 Subunit
    • Core Binding Factor Alpha 3 Subunit
    • Cytoskeletal Proteins
    • DNA-Binding Proteins
    • Epstein-Barr Virus Nuclear Antigens
    • Gene Expression Profiling
    • Herpesvirus 4, Human
    • Humans
    • Interleukin-16
    • Intracellular Signaling Peptides and Proteins
    • LIM Domain Proteins
    • Lymphocyte Activation
    • Oligonucleotide Array Sequence Analysis
    • Phenotype
    • Proto-Oncogene Proteins
    • Transcription Factors
    • Tumor Cells, Cultured
    • Virus Latency
    • Comparative Study
    • Journal Article

    Cite this

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    title = "Expression of transcription factor AML-2 (RUNX3, CBF(alpha)-3) is induced by Epstein-Barr virus EBNA-2 and correlates with the B-cell activation phenotype",
    abstract = "To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.",
    keywords = "Adaptor Proteins, Signal Transducing, B-Lymphocytes, Burkitt Lymphoma, Carrier Proteins, Cell Line, Cell Transformation, Viral, Cells, Cultured, Core Binding Factor Alpha 2 Subunit, Core Binding Factor Alpha 3 Subunit, Cytoskeletal Proteins, DNA-Binding Proteins, Epstein-Barr Virus Nuclear Antigens, Gene Expression Profiling, Herpesvirus 4, Human, Humans, Interleukin-16, Intracellular Signaling Peptides and Proteins, LIM Domain Proteins, Lymphocyte Activation, Oligonucleotide Array Sequence Analysis, Phenotype, Proto-Oncogene Proteins, Transcription Factors, Tumor Cells, Cultured, Virus Latency, Comparative Study, Journal Article",
    author = "Spender, {Lindsay C} and Cornish, {Georgina H} and Alexandra Sullivan and Farrell, {Paul J}",
    year = "2002",
    month = "5",
    doi = "10.1128/JVI.76.10.4919-4927.2002",
    language = "English",
    volume = "76",
    pages = "4919--4927",
    journal = "Journal of Virology",
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    publisher = "American Society for Microbiology",
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    }

    Expression of transcription factor AML-2 (RUNX3, CBF(alpha)-3) is induced by Epstein-Barr virus EBNA-2 and correlates with the B-cell activation phenotype. / Spender, Lindsay C; Cornish, Georgina H; Sullivan, Alexandra; Farrell, Paul J (Lead / Corresponding author).

    In: Journal of Virology, Vol. 76, No. 10, 05.2002, p. 4919-4927.

    Research output: Contribution to journalArticle

    TY - JOUR

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    AU - Spender, Lindsay C

    AU - Cornish, Georgina H

    AU - Sullivan, Alexandra

    AU - Farrell, Paul J

    PY - 2002/5

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    N2 - To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.

    AB - To identify cell proteins regulated by the Epstein-Barr virus (EBV) transcription factor EBNA-2, we analyzed a cell line with conditional EBNA-2 activity by using microarray expression profiling. This led to the identification of two novel target genes induced by EBNA-2. The first of these, interleukin-16, is an immunomodulatory cytokine involved in the regulation of CD4 T cells. The second, AML-2, is a member of the Runt domain family of transcription factors. Quiescent B cells initially expressed AML-1 but, 48 h after virus infection, the levels of AML-1 decreased dramatically, whereas the amount of AML-2 protein increased. Analysis of a panel of B-cell lines indicated that AML-2 expression is normally predominant in EBV latency III, whereas AML-1 is associated with EBV latency I or EBV-negative cells. The AML genes are the first example of cell transcription factors whose expression correlates with the latency I/III phenotype.

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    KW - Burkitt Lymphoma

    KW - Carrier Proteins

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    KW - Cell Transformation, Viral

    KW - Cells, Cultured

    KW - Core Binding Factor Alpha 2 Subunit

    KW - Core Binding Factor Alpha 3 Subunit

    KW - Cytoskeletal Proteins

    KW - DNA-Binding Proteins

    KW - Epstein-Barr Virus Nuclear Antigens

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    KW - Herpesvirus 4, Human

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    KW - Lymphocyte Activation

    KW - Oligonucleotide Array Sequence Analysis

    KW - Phenotype

    KW - Proto-Oncogene Proteins

    KW - Transcription Factors

    KW - Tumor Cells, Cultured

    KW - Virus Latency

    KW - Comparative Study

    KW - Journal Article

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    JO - Journal of Virology

    JF - Journal of Virology

    SN - 0022-538X

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    ER -