Extracellular metal-binding activity of the sulphate-reducing bacterium Desulfococcus multivorans

Toni A. M. Bridge, Chris White, Geoffrey M. Gadd (Lead / Corresponding author)

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    64 Citations (Scopus)

    Abstract

    Polarography was used to measure the copper-binding ability of culture filtrates from a range of sulphate-reducing bacteria (SRB), including pure cultures and environmental isolates. Of those tested, Desulfococcus multivorans was shown to have the greatest copper-binding capacity and this organism was used for further experiments. Extracellular copper- and zinc-binding activities of Dc. multivorans culture filtrates from batch cultures increased over time and reached a maximum after 10 d growth. The culture filtrate was shown to bind copper reversibly and zinc irreversibly. Twelve-day-old Dc. multivorans culture filtrates were shown to have a copper-binding capacity of 3.64 ± 0.33 μmol ml-1 with a stability constant, log10K, of 5.68 ± 0.64 (n = 4). The metal-binding compound was partially purified from culture growth media by dichloromethane extraction followed by HPLC using an acetonitrile gradient.

    Original languageEnglish
    Pages (from-to)2987-2995
    Number of pages9
    JournalMicrobiology
    Volume145
    Issue number10
    DOIs
    Publication statusPublished - 1 Oct 1999

    Keywords

    • Copper binding
    • Desulfococcus multivorans
    • Metal binding
    • Sulphate-reducing bacteria
    • Zinc binding
    • SRB
    • sulphate-reducing bacteria

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