Extracellular metal-binding activity of the sulphate-reducing bacterium Desulfococcus multivorans

TY - JOUR

T1 - Extracellular metal-binding activity of the sulphate-reducing bacterium Desulfococcus multivorans

AU - Bridge, Toni A. M.

AU - White, Chris

AU - Gadd, Geoffrey M.

PY - 1999/10/1

Y1 - 1999/10/1

N2 - Polarography was used to measure the copper-binding ability of culture filtrates from a range of sulphate-reducing bacteria (SRB), including pure cultures and environmental isolates. Of those tested, Desulfococcus multivorans was shown to have the greatest copper-binding capacity and this organism was used for further experiments. Extracellular copper- and zinc-binding activities of Dc. multivorans culture filtrates from batch cultures increased over time and reached a maximum after 10 d growth. The culture filtrate was shown to bind copper reversibly and zinc irreversibly. Twelve-day-old Dc. multivorans culture filtrates were shown to have a copper-binding capacity of 3.64 ± 0.33 μmol ml-1 with a stability constant, log10K, of 5.68 ± 0.64 (n = 4). The metal-binding compound was partially purified from culture growth media by dichloromethane extraction followed by HPLC using an acetonitrile gradient.

AB - Polarography was used to measure the copper-binding ability of culture filtrates from a range of sulphate-reducing bacteria (SRB), including pure cultures and environmental isolates. Of those tested, Desulfococcus multivorans was shown to have the greatest copper-binding capacity and this organism was used for further experiments. Extracellular copper- and zinc-binding activities of Dc. multivorans culture filtrates from batch cultures increased over time and reached a maximum after 10 d growth. The culture filtrate was shown to bind copper reversibly and zinc irreversibly. Twelve-day-old Dc. multivorans culture filtrates were shown to have a copper-binding capacity of 3.64 ± 0.33 μmol ml-1 with a stability constant, log10K, of 5.68 ± 0.64 (n = 4). The metal-binding compound was partially purified from culture growth media by dichloromethane extraction followed by HPLC using an acetonitrile gradient.

KW - Copper binding

KW - Desulfococcus multivorans

KW - Metal binding

KW - Sulphate-reducing bacteria

KW - Zinc binding

KW - SRB

KW - sulphate-reducing bacteria

UR - https://www.scopus.com/pages/publications/0032850587

U2 - 10.1099/00221287-145-10-2987

DO - 10.1099/00221287-145-10-2987

M3 - Article

C2 - 10537221

AN - SCOPUS:0032850587

SN - 1350-0872

VL - 145

SP - 2987

EP - 2995

JO - Microbiology

JF - Microbiology

IS - 10

ER -