F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil

J. W. Hong; M. Fomina; G. M. Gadd

doi:10.1111/j.1365-2672.2009.04665.x

F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil

J. W. Hong, M. Fomina, G. M. Gadd

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Aims:

This work has examined the effects of a polycyclic aromatic hydrocarbon and selected toxic metals on fungal populations in a soil microcosm.

Methods and Results:

By using fungal ribosomal intergenic spacer analysis (F-RISA) in combination with real-time PCR quantification, four fungi (D63P2-1, D63C2-1, D21Cu1-1 and D63Pb2-2) with specific primer pairs to each were successfully evaluated for their potential as bioindicators in response to pyrene, copper (Cu) and lead (Pb), supplied singly and in combination.

Conclusions:

F-RISA coupled with real-time PCR is a useful approach for the identification of microorganisms with potential as bioindicators of organic and toxic metal contamination.

Significance and Impact of the Study:

These bioindicators could be monitored for their population changes that may indicate pollutant-induced perturbations in a given system.

Original language	English
Pages (from-to)	415-430
Number of pages	16
Journal	Journal of Applied Microbiology
Volume	109
Issue number	2
DOIs	https://doi.org/10.1111/j.1365-2672.2009.04665.x
Publication status	Published - Aug 2010

Keywords

bioindicator
F-RISA
fungi
organic and metal contamination
real-time PCR
COMMUNITY STRUCTURE
AGRICULTURAL SOILS
HUMIC SUBSTANCES
MICROORGANISMS
DNA
STERILIZATION
MINERALS
SAMPLES
TRANSFORMATIONS
DEGRADATION

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10.1111/j.1365-2672.2009.04665.x

Cite this

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title = "F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil",

abstract = "Aims:This work has examined the effects of a polycyclic aromatic hydrocarbon and selected toxic metals on fungal populations in a soil microcosm.Methods and Results:By using fungal ribosomal intergenic spacer analysis (F-RISA) in combination with real-time PCR quantification, four fungi (D63P2-1, D63C2-1, D21Cu1-1 and D63Pb2-2) with specific primer pairs to each were successfully evaluated for their potential as bioindicators in response to pyrene, copper (Cu) and lead (Pb), supplied singly and in combination.Conclusions:F-RISA coupled with real-time PCR is a useful approach for the identification of microorganisms with potential as bioindicators of organic and toxic metal contamination.Significance and Impact of the Study:These bioindicators could be monitored for their population changes that may indicate pollutant-induced perturbations in a given system.",

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author = "Hong, {J. W.} and M. Fomina and Gadd, {G. M.}",

year = "2010",

month = aug,

doi = "10.1111/j.1365-2672.2009.04665.x",

language = "English",

volume = "109",

pages = "415--430",

journal = "Journal of Applied Microbiology",

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TY - JOUR

T1 - F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil

AU - Hong, J. W.

AU - Fomina, M.

AU - Gadd, G. M.

PY - 2010/8

Y1 - 2010/8

N2 - Aims:This work has examined the effects of a polycyclic aromatic hydrocarbon and selected toxic metals on fungal populations in a soil microcosm.Methods and Results:By using fungal ribosomal intergenic spacer analysis (F-RISA) in combination with real-time PCR quantification, four fungi (D63P2-1, D63C2-1, D21Cu1-1 and D63Pb2-2) with specific primer pairs to each were successfully evaluated for their potential as bioindicators in response to pyrene, copper (Cu) and lead (Pb), supplied singly and in combination.Conclusions:F-RISA coupled with real-time PCR is a useful approach for the identification of microorganisms with potential as bioindicators of organic and toxic metal contamination.Significance and Impact of the Study:These bioindicators could be monitored for their population changes that may indicate pollutant-induced perturbations in a given system.

AB - Aims:This work has examined the effects of a polycyclic aromatic hydrocarbon and selected toxic metals on fungal populations in a soil microcosm.Methods and Results:By using fungal ribosomal intergenic spacer analysis (F-RISA) in combination with real-time PCR quantification, four fungi (D63P2-1, D63C2-1, D21Cu1-1 and D63Pb2-2) with specific primer pairs to each were successfully evaluated for their potential as bioindicators in response to pyrene, copper (Cu) and lead (Pb), supplied singly and in combination.Conclusions:F-RISA coupled with real-time PCR is a useful approach for the identification of microorganisms with potential as bioindicators of organic and toxic metal contamination.Significance and Impact of the Study:These bioindicators could be monitored for their population changes that may indicate pollutant-induced perturbations in a given system.

KW - bioindicator

KW - F-RISA

KW - fungi

KW - organic and metal contamination

KW - real-time PCR

KW - COMMUNITY STRUCTURE

KW - AGRICULTURAL SOILS

KW - HUMIC SUBSTANCES

KW - MICROORGANISMS

KW - DNA

KW - STERILIZATION

KW - MINERALS

KW - SAMPLES

KW - TRANSFORMATIONS

KW - DEGRADATION

U2 - 10.1111/j.1365-2672.2009.04665.x

DO - 10.1111/j.1365-2672.2009.04665.x

M3 - Article

C2 - 20092541

SN - 1364-5072

VL - 109

SP - 415

EP - 430

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

IS - 2

ER -

F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil

Abstract

Keywords

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