F-RISA fungal clones as potential bioindicators of organic and metal contamination in soil

J. W. Hong, M. Fomina, G. M. Gadd

    Research output: Contribution to journalArticle

    8 Citations (Scopus)

    Abstract

    Aims:

    This work has examined the effects of a polycyclic aromatic hydrocarbon and selected toxic metals on fungal populations in a soil microcosm.

    Methods and Results:

    By using fungal ribosomal intergenic spacer analysis (F-RISA) in combination with real-time PCR quantification, four fungi (D63P2-1, D63C2-1, D21Cu1-1 and D63Pb2-2) with specific primer pairs to each were successfully evaluated for their potential as bioindicators in response to pyrene, copper (Cu) and lead (Pb), supplied singly and in combination.

    Conclusions:

    F-RISA coupled with real-time PCR is a useful approach for the identification of microorganisms with potential as bioindicators of organic and toxic metal contamination.

    Significance and Impact of the Study:

    These bioindicators could be monitored for their population changes that may indicate pollutant-induced perturbations in a given system.

    Original languageEnglish
    Pages (from-to)415-430
    Number of pages16
    JournalJournal of Applied Microbiology
    Volume109
    Issue number2
    DOIs
    Publication statusPublished - Aug 2010

    Keywords

    • bioindicator
    • F-RISA
    • fungi
    • organic and metal contamination
    • real-time PCR
    • COMMUNITY STRUCTURE
    • AGRICULTURAL SOILS
    • HUMIC SUBSTANCES
    • MICROORGANISMS
    • DNA
    • STERILIZATION
    • MINERALS
    • SAMPLES
    • TRANSFORMATIONS
    • DEGRADATION

    Cite this