Fabrication of agar-based tissue-mimicking phantom for the technical evaluation of biomedical optical imaging systems

Mingyu Kim, Seonghui Im, Inyoung Park, Donghyeok Kim, Eun Su Kim, James Joseph, Jonghee Yoon (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
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The development process of the optical systems for various biomedical applications typically involve evaluations of technical performance. One popular evaluation method is to use a reference object such as a phantom that exhibits similar optical properties of tissue. Fabrication of a consistent phantom with known optical properties, such as scattering and absorption, is essential for accurate technical evaluation of the optical system. This paper presents a protocol for fabricating an agar-based tissue-mimicking phantom, offering practical guidance to ensure consistent and reproducible phantom creation. In addition, optical setups that measure light information required for quantifying the optical properties via an inverse adding-doubling (IAD) method are discussed. We demonstrated the fabrication of phantoms with diverse scattering and absorption properties, and the IAD method successfully quantified the optical properties. Moreover, we employed the phantom to assess the imaging depth limitation of a hyperspectral imaging system, demonstrating potential usage of phantoms for performing technical evaluation.

Original languageEnglish
Pages (from-to)80-85
Number of pages6
JournalCurrent Applied Physics
Early online date28 Feb 2024
Publication statusPublished - May 2024


  • Light-tissue interaction
  • Optical system
  • Soft tissue
  • Technical evaluation
  • Tissue-mimicking phantom

ASJC Scopus subject areas

  • General Materials Science
  • General Physics and Astronomy


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