Fabrication of antibacterial Fe3+-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters

Jian-xiang Li; Hong Yang; Rong-hua Gu; Yu-hang Dong; Yong-wei Cai; Qi Zhao; Yao Chen; Mi Huang

doi:10.1016/j.colsurfa.2023.131680

Fabrication of antibacterial Fe³⁺-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters

Jian-xiang Li
, Hong Yang
, Rong-hua Gu
, Yu-hang Dong
, Yong-wei Cai (Lead / Corresponding author)
, Qi Zhao (Lead / Corresponding author)
, Yao Chen
, Mi Huang

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

204 Downloads (Pure)

Abstract

Catheter-associated urinary tract infections (CAUTIs) are one of the most common hospital-acquired infections. In this study, a new Fe³⁺-carboxymethylcellulose (CMC)-polyacrylamide (PAAm) - Ag nanoparticles (AgNPs) interpenetrating hydrogel coating with antibacterial properties on the surfaces of latex urinary catheters was prepared with a simple dipping method. The morphology, chemical composition, surface roughness, and contact angles of the coated urinary catheters were characterized by using SEM, EDS, ATR-FTIR, XPS, AFM, and contact angle instruments, respectively. The antibacterial performance of the coated urinary catheters was characterized by the inhibition zone tests. Furthermore, the toxicity profile of the coated urinary catheters was also evaluated in vitro via MTT assay and hemolysis test. The experimental results showed that the Fe³⁺-CMC-PAAm-AgNPs hydrogel-coated urinary catheters had strong antibacterial effects. The concentration of AgNPs after the release of 30 days was still higher than the effective antibacterial concentration of the total Ag (0.1 ppb) and was lower than the toxic concentration of human cells (10 ppm). The inhibition zone tests of the coated urinary catheter against E. coli (ATCC25922) showed that both the CMC-PAAm-AgNPs hydrogel coatings and the Fe³⁺-CMC-PAAm-AgNPs hydrogel coatings had clear inhibition zones in the culture medium. The addition of Fe³⁺ exhibited synergistic antibacterial effects with AgNPs. Moreover, the coatings also showed admirable biocompatibility and hemocompatibility. The synergistic antibacterial mechanism was also proposed. The novel Fe³⁺-CMC-PAAm-AgNPs hydrogel coatings for urinary catheters would have great potential in reducing CAUTIs.

Original language	English
Article number	131680
Number of pages	12
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	672
Early online date	18 May 2023
DOIs	https://doi.org/10.1016/j.colsurfa.2023.131680
Publication status	Published - 5 Sept 2023

Keywords

AgNPs
Antibacterial coating
Double-network hydrogel
Fe
Polyacrylamide
Urinary catheter

ASJC Scopus subject areas

Surfaces and Interfaces
Physical and Theoretical Chemistry
Colloid and Surface Chemistry

Access to Document

10.1016/j.colsurfa.2023.131680

Author Accepted ManuscriptAccepted author manuscript, 2.35 MBLicence: CC BY-NC-ND

Influence of Surface Properties of New Biomaterials for Catheters on Bacterial Adhesion Urine
Campbell, P. (Investigator), Corner, G. (Investigator), Davidson, F. (Investigator), Keatch, R. (Investigator), Nabi, G. (Investigator), Vorstius, J. B. (Investigator), Wilcox, K. (Investigator) & Zhao, Q. (Investigator)
1/12/16 → 31/10/21
Project: Research

Cite this

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title = "Fabrication of antibacterial Fe3+-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters",

abstract = "Catheter-associated urinary tract infections (CAUTIs) are one of the most common hospital-acquired infections. In this study, a new Fe3+-carboxymethylcellulose (CMC)-polyacrylamide (PAAm) - Ag nanoparticles (AgNPs) interpenetrating hydrogel coating with antibacterial properties on the surfaces of latex urinary catheters was prepared with a simple dipping method. The morphology, chemical composition, surface roughness, and contact angles of the coated urinary catheters were characterized by using SEM, EDS, ATR-FTIR, XPS, AFM, and contact angle instruments, respectively. The antibacterial performance of the coated urinary catheters was characterized by the inhibition zone tests. Furthermore, the toxicity profile of the coated urinary catheters was also evaluated in vitro via MTT assay and hemolysis test. The experimental results showed that the Fe3+-CMC-PAAm-AgNPs hydrogel-coated urinary catheters had strong antibacterial effects. The concentration of AgNPs after the release of 30 days was still higher than the effective antibacterial concentration of the total Ag (0.1 ppb) and was lower than the toxic concentration of human cells (10 ppm). The inhibition zone tests of the coated urinary catheter against E. coli (ATCC25922) showed that both the CMC-PAAm-AgNPs hydrogel coatings and the Fe3+-CMC-PAAm-AgNPs hydrogel coatings had clear inhibition zones in the culture medium. The addition of Fe3+ exhibited synergistic antibacterial effects with AgNPs. Moreover, the coatings also showed admirable biocompatibility and hemocompatibility. The synergistic antibacterial mechanism was also proposed. The novel Fe3+-CMC-PAAm-AgNPs hydrogel coatings for urinary catheters would have great potential in reducing CAUTIs.",

keywords = "AgNPs, Antibacterial coating, Double-network hydrogel, Fe, Polyacrylamide, Urinary catheter",

author = "Jian-xiang Li and Hong Yang and Rong-hua Gu and Yu-hang Dong and Yong-wei Cai and Qi Zhao and Yao Chen and Mi Huang",

year = "2023",

month = sep,

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doi = "10.1016/j.colsurfa.2023.131680",

language = "English",

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journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",

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T1 - Fabrication of antibacterial Fe3+-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters

AU - Li, Jian-xiang

AU - Yang, Hong

AU - Gu, Rong-hua

AU - Dong, Yu-hang

AU - Cai, Yong-wei

AU - Zhao, Qi

AU - Chen, Yao

AU - Huang, Mi

PY - 2023/9/5

Y1 - 2023/9/5

N2 - Catheter-associated urinary tract infections (CAUTIs) are one of the most common hospital-acquired infections. In this study, a new Fe3+-carboxymethylcellulose (CMC)-polyacrylamide (PAAm) - Ag nanoparticles (AgNPs) interpenetrating hydrogel coating with antibacterial properties on the surfaces of latex urinary catheters was prepared with a simple dipping method. The morphology, chemical composition, surface roughness, and contact angles of the coated urinary catheters were characterized by using SEM, EDS, ATR-FTIR, XPS, AFM, and contact angle instruments, respectively. The antibacterial performance of the coated urinary catheters was characterized by the inhibition zone tests. Furthermore, the toxicity profile of the coated urinary catheters was also evaluated in vitro via MTT assay and hemolysis test. The experimental results showed that the Fe3+-CMC-PAAm-AgNPs hydrogel-coated urinary catheters had strong antibacterial effects. The concentration of AgNPs after the release of 30 days was still higher than the effective antibacterial concentration of the total Ag (0.1 ppb) and was lower than the toxic concentration of human cells (10 ppm). The inhibition zone tests of the coated urinary catheter against E. coli (ATCC25922) showed that both the CMC-PAAm-AgNPs hydrogel coatings and the Fe3+-CMC-PAAm-AgNPs hydrogel coatings had clear inhibition zones in the culture medium. The addition of Fe3+ exhibited synergistic antibacterial effects with AgNPs. Moreover, the coatings also showed admirable biocompatibility and hemocompatibility. The synergistic antibacterial mechanism was also proposed. The novel Fe3+-CMC-PAAm-AgNPs hydrogel coatings for urinary catheters would have great potential in reducing CAUTIs.

AB - Catheter-associated urinary tract infections (CAUTIs) are one of the most common hospital-acquired infections. In this study, a new Fe3+-carboxymethylcellulose (CMC)-polyacrylamide (PAAm) - Ag nanoparticles (AgNPs) interpenetrating hydrogel coating with antibacterial properties on the surfaces of latex urinary catheters was prepared with a simple dipping method. The morphology, chemical composition, surface roughness, and contact angles of the coated urinary catheters were characterized by using SEM, EDS, ATR-FTIR, XPS, AFM, and contact angle instruments, respectively. The antibacterial performance of the coated urinary catheters was characterized by the inhibition zone tests. Furthermore, the toxicity profile of the coated urinary catheters was also evaluated in vitro via MTT assay and hemolysis test. The experimental results showed that the Fe3+-CMC-PAAm-AgNPs hydrogel-coated urinary catheters had strong antibacterial effects. The concentration of AgNPs after the release of 30 days was still higher than the effective antibacterial concentration of the total Ag (0.1 ppb) and was lower than the toxic concentration of human cells (10 ppm). The inhibition zone tests of the coated urinary catheter against E. coli (ATCC25922) showed that both the CMC-PAAm-AgNPs hydrogel coatings and the Fe3+-CMC-PAAm-AgNPs hydrogel coatings had clear inhibition zones in the culture medium. The addition of Fe3+ exhibited synergistic antibacterial effects with AgNPs. Moreover, the coatings also showed admirable biocompatibility and hemocompatibility. The synergistic antibacterial mechanism was also proposed. The novel Fe3+-CMC-PAAm-AgNPs hydrogel coatings for urinary catheters would have great potential in reducing CAUTIs.

KW - AgNPs

KW - Antibacterial coating

KW - Double-network hydrogel

KW - Fe

KW - Polyacrylamide

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U2 - 10.1016/j.colsurfa.2023.131680

DO - 10.1016/j.colsurfa.2023.131680

M3 - Article

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SN - 0927-7757

VL - 672

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

M1 - 131680

ER -