Factors affecting the longevity and strength in an In Vitro model of the bone-ligament interface

Jennifer Z. Paxton; Kenneth Donnelly; Robert P. Keatch; Keith Baar; Liam M. Grover

doi:10.1007/s10439-010-0044-0

Factors affecting the longevity and strength in an In Vitro model of the bone-ligament interface

Jennifer Z. Paxton, Kenneth Donnelly, Robert P. Keatch, Keith Baar, Liam M. Grover

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

The interfaces between musculoskeletal tissues with contrasting moduli are morphologically and biochemically adapted to allow the transmission of force with minimal injury. Current methods of tissue engineering ligaments and tendons do not include the interface and this may limit the future clinical success of engineered musculoskeletal tissues. This study aimed to use solid brushite cement anchors to engineer intact ligaments from bone-to-bone, creating a functional musculoskeletal interface in vitro. We show here that modifying anchor shape and cement composition can alter both the longevity and the strength of an in vitro model of the bone-ligament interface: with values reaching 23 days and 21.6 kPa, respectively. These results validate the use of brushite bone cement to engineer the bone-ligament interface in vitro and raise the potential for future use in ligament replacement surgery.

Original language	English
Pages (from-to)	2155-2166
Number of pages	12
Journal	Annals of Biomedical Engineering
Volume	38
Issue number	6
DOIs	https://doi.org/10.1007/s10439-010-0044-0
Publication status	Published - Jun 2010

Keywords

Ligament
Enthesis
Regenerative medicine
Brushite
CALCIUM-PHOSPHATE CEMENT
DONOR-SITE MORBIDITY
BRUSHITE CEMENT
RECONSTRUCTION
HYDROXYAPATITE
AUTOGRAFT
SCAFFOLD
PYROPHOSPHATE
INTEGRATION
CONSTRUCTS

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abstract = "The interfaces between musculoskeletal tissues with contrasting moduli are morphologically and biochemically adapted to allow the transmission of force with minimal injury. Current methods of tissue engineering ligaments and tendons do not include the interface and this may limit the future clinical success of engineered musculoskeletal tissues. This study aimed to use solid brushite cement anchors to engineer intact ligaments from bone-to-bone, creating a functional musculoskeletal interface in vitro. We show here that modifying anchor shape and cement composition can alter both the longevity and the strength of an in vitro model of the bone-ligament interface: with values reaching 23 days and 21.6 kPa, respectively. These results validate the use of brushite bone cement to engineer the bone-ligament interface in vitro and raise the potential for future use in ligament replacement surgery.",

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AU - Donnelly, Kenneth

AU - Keatch, Robert P.

AU - Baar, Keith

AU - Grover, Liam M.

PY - 2010/6

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N2 - The interfaces between musculoskeletal tissues with contrasting moduli are morphologically and biochemically adapted to allow the transmission of force with minimal injury. Current methods of tissue engineering ligaments and tendons do not include the interface and this may limit the future clinical success of engineered musculoskeletal tissues. This study aimed to use solid brushite cement anchors to engineer intact ligaments from bone-to-bone, creating a functional musculoskeletal interface in vitro. We show here that modifying anchor shape and cement composition can alter both the longevity and the strength of an in vitro model of the bone-ligament interface: with values reaching 23 days and 21.6 kPa, respectively. These results validate the use of brushite bone cement to engineer the bone-ligament interface in vitro and raise the potential for future use in ligament replacement surgery.

AB - The interfaces between musculoskeletal tissues with contrasting moduli are morphologically and biochemically adapted to allow the transmission of force with minimal injury. Current methods of tissue engineering ligaments and tendons do not include the interface and this may limit the future clinical success of engineered musculoskeletal tissues. This study aimed to use solid brushite cement anchors to engineer intact ligaments from bone-to-bone, creating a functional musculoskeletal interface in vitro. We show here that modifying anchor shape and cement composition can alter both the longevity and the strength of an in vitro model of the bone-ligament interface: with values reaching 23 days and 21.6 kPa, respectively. These results validate the use of brushite bone cement to engineer the bone-ligament interface in vitro and raise the potential for future use in ligament replacement surgery.

KW - Ligament

KW - Enthesis

KW - Regenerative medicine

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KW - DONOR-SITE MORBIDITY

KW - BRUSHITE CEMENT

KW - RECONSTRUCTION

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KW - AUTOGRAFT

KW - SCAFFOLD

KW - PYROPHOSPHATE

KW - INTEGRATION

KW - CONSTRUCTS

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JO - Annals of Biomedical Engineering

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IS - 6

ER -

Factors affecting the longevity and strength in an In Vitro model of the bone-ligament interface

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Keywords

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