Projects per year
Abstract
The concerted action of many protein kinases helps orchestrate the error-free progression through mitosis of mammalian cells. The roles and regulation of some prominent mitotic kinases, such as cyclin-dependent kinases, are well established. However, these and other known mitotic kinases alone cannot account for the extent of protein phosphorylation that has been reported during mammalian mitosis. Here we demonstrate that CK1α, of the casein kinase 1 family of protein kinases, localises to the spindle and is required for proper spindle positioning and timely cell division. CK1α is recruited to the spindle by FAM83D, and cells devoid of FAM83D, or those harbouring CK1α-binding-deficient FAM83D F283A/F283A knockin mutations, display pronounced spindle positioning defects, and a prolonged mitosis. Restoring FAM83D at the endogenous locus in FAM83D −/− cells, or artificially delivering CK1α to the spindle in FAM83D F283A/F283A cells, rescues these defects. These findings implicate CK1α as new mitotic kinase that orchestrates the kinetics and orientation of cell division.
Original language | English |
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Article number | e47495 |
Pages (from-to) | 1-19 |
Number of pages | 19 |
Journal | EMBO Reports |
Volume | 20 |
Issue number | 9 |
Early online date | 24 Jul 2019 |
DOIs | |
Publication status | Published - 1 Sept 2019 |
Keywords
- CK1
- FAM83D
- kinase
- mitosis
- spindle positioning
ASJC Scopus subject areas
- Genetics
- Molecular Biology
- Biochemistry
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Dive into the research topics of 'FAM83D directs protein kinase CK1α to the mitotic spindle for proper spindle positioning'. Together they form a unique fingerprint.Projects
- 1 Finished
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Dynamics of Fundamental Cellular Processes by Live Cell and Tissue Imaging
MacDonald, M. (Investigator), McGloin, D. (Investigator), McKenna, S. (Investigator), Storey, K. (Investigator), Swedlow, J. (Investigator) & Weijer, K. (Investigator)
1/01/13 → 31/12/17
Project: Research
Student theses
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Identification and characterisation of FAM83 proteins as key regulators of CK1 protein kinases in cells: FAM83D recruits CK1α to the mitotic spindle for proper spindle positioning
Fulcher, L. J. (Author), Sapkota, G. (Supervisor) & Ganley, I. (Supervisor), 2019Student thesis: Doctoral Thesis › Doctor of Philosophy
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