FAM83F regulates canonical Wnt signalling through an interaction with CK1α

Karen Dunbar, Rebecca A. Jones, Kevin Dingwell, Thomas J. Macartney, James C. Smith, Gopal Sapkota (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

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Abstract

The function of the FAM83F protein, like the functions of many members of the FAM83 family, is poorly understood. Here, we show that injection of Fam83f mRNA into Xenopus embryos causes axis duplication, a phenotype indicative of enhanced Wnt signalling. Consistent with this, overexpression of FAM83F activates Wnt signalling, whereas ablation of FAM83F from human colorectal cancer (CRC) cells attenuates it. We demonstrate that FAM83F is farnesylated and interacts and co-localises with CK1α at the plasma membrane. This interaction with CK1α is essential for FAM83F to activate Wnt signalling, and FAM83F mutants that do not interact with CK1α fail to induce axis duplication in Xenopus embryos and to activate Wnt signalling in cells. FAM83F acts upstream of GSK-3β because the attenuation of Wnt signalling caused by loss of FAM83F can be rescued by GSK-3 inhibition. Introduction of a farnesyl-deficient mutant of FAM83F in cells through CRISPR/Cas9 genome editing redirects the FAM83F-CK1α complex away from the plasma membrane and significantly attenuates Wnt signalling, indicating that FAM83F exerts its effects on Wnt signalling at the plasma membrane.

Original languageEnglish
Article numbere202000805
Number of pages17
JournalLife Science Alliance
Volume4
Issue number2
Early online date24 Dec 2020
DOIs
Publication statusPublished - Feb 2021

Keywords

  • Colorectal cancer
  • Xenopus axis duplication
  • farnesylation
  • plasma membrane

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