Family with Sequence Similarity 60A (FAM60A) Protein Is a Cell Cycle-fluctuating Regulator of the SIN3-HDAC1 Histone Deacetylase Complex

Ivan M. Munoz, Thomas Macartney, Luis Sanchez-Pulido, Chris P. Ponting, Sonia Rocha, John Rouse

    Research output: Contribution to journalArticle

    17 Citations (Scopus)

    Abstract

    The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. Here we describe family with sequence similarity 60A (FAM60A), a cell cycle-regulated protein that binds to the SIN3-HDAC complex. FAM60A expression peaks during G(1) and S phases of the cell cycle in U2OS cells, in a manner similar to the G(1) regulator cyclin D1, which is a known target of SIN3-HDAC. In this light we found that FAM60A binds to SIN3-HDAC-regulated promoters such as cyclin D1 in G(1) and S phases. Cells depleted of FAM60A show increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry. The data in this study introduce FAM60A as a novel regulator of SIN3-HDAC function and gene expression.
    Original languageEnglish
    Pages (from-to)32346-53
    Number of pages8
    JournalJournal of Biological Chemistry
    Volume287
    Issue number39
    DOIs
    Publication statusPublished - 21 Sep 2012

    Fingerprint

    Sin3 Histone Deacetylase and Corepressor Complex
    Histone Deacetylase 1
    Histone Deacetylases
    Cyclin D1
    Cell Cycle
    Cells
    S Phase
    Proteins
    Histones
    Acetylation
    Cell Cycle Proteins
    Transcription
    Gene expression
    Genes
    Association reactions
    Gene Expression
    Messenger RNA

    Cite this

    @article{3e2cfcc428f94d3bb70ae1108620c789,
    title = "Family with Sequence Similarity 60A (FAM60A) Protein Is a Cell Cycle-fluctuating Regulator of the SIN3-HDAC1 Histone Deacetylase Complex",
    abstract = "The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. Here we describe family with sequence similarity 60A (FAM60A), a cell cycle-regulated protein that binds to the SIN3-HDAC complex. FAM60A expression peaks during G(1) and S phases of the cell cycle in U2OS cells, in a manner similar to the G(1) regulator cyclin D1, which is a known target of SIN3-HDAC. In this light we found that FAM60A binds to SIN3-HDAC-regulated promoters such as cyclin D1 in G(1) and S phases. Cells depleted of FAM60A show increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry. The data in this study introduce FAM60A as a novel regulator of SIN3-HDAC function and gene expression.",
    author = "Munoz, {Ivan M.} and Thomas Macartney and Luis Sanchez-Pulido and Ponting, {Chris P.} and Sonia Rocha and John Rouse",
    year = "2012",
    month = "9",
    day = "21",
    doi = "10.1074/jbc.M112.382499",
    language = "English",
    volume = "287",
    pages = "32346--53",
    journal = "Journal of Biological Chemistry",
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    Family with Sequence Similarity 60A (FAM60A) Protein Is a Cell Cycle-fluctuating Regulator of the SIN3-HDAC1 Histone Deacetylase Complex. / Munoz, Ivan M.; Macartney, Thomas; Sanchez-Pulido, Luis; Ponting, Chris P.; Rocha, Sonia; Rouse, John.

    In: Journal of Biological Chemistry, Vol. 287, No. 39, 21.09.2012, p. 32346-53.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Family with Sequence Similarity 60A (FAM60A) Protein Is a Cell Cycle-fluctuating Regulator of the SIN3-HDAC1 Histone Deacetylase Complex

    AU - Munoz, Ivan M.

    AU - Macartney, Thomas

    AU - Sanchez-Pulido, Luis

    AU - Ponting, Chris P.

    AU - Rocha, Sonia

    AU - Rouse, John

    PY - 2012/9/21

    Y1 - 2012/9/21

    N2 - The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. Here we describe family with sequence similarity 60A (FAM60A), a cell cycle-regulated protein that binds to the SIN3-HDAC complex. FAM60A expression peaks during G(1) and S phases of the cell cycle in U2OS cells, in a manner similar to the G(1) regulator cyclin D1, which is a known target of SIN3-HDAC. In this light we found that FAM60A binds to SIN3-HDAC-regulated promoters such as cyclin D1 in G(1) and S phases. Cells depleted of FAM60A show increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry. The data in this study introduce FAM60A as a novel regulator of SIN3-HDAC function and gene expression.

    AB - The SIN3A-HDAC complex deacetylates histones thereby repressing gene transcription. Here we describe family with sequence similarity 60A (FAM60A), a cell cycle-regulated protein that binds to the SIN3-HDAC complex. FAM60A expression peaks during G(1) and S phases of the cell cycle in U2OS cells, in a manner similar to the G(1) regulator cyclin D1, which is a known target of SIN3-HDAC. In this light we found that FAM60A binds to SIN3-HDAC-regulated promoters such as cyclin D1 in G(1) and S phases. Cells depleted of FAM60A show increased histone acetylation at the cyclin D1 promoter and elevated levels of cyclin D1 mRNA and protein. Furthermore, depletion of FAM60A altered the periodic association of HDAC1 with the cyclin D1 promoter, increased cyclin D1 expression at all cell cycle phases, and caused premature S phase entry. The data in this study introduce FAM60A as a novel regulator of SIN3-HDAC function and gene expression.

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