Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species

Mattias C. Gustafsson, Colin N. Palmer, Roland C. Wolf, Claes von Wachenfeldt

    Research output: Contribution to journalArticle

    21 Citations (Scopus)

    Abstract

    Bacillus subtilis strain 168 encodes two flavocytochromes P450, Cyp102A2 and Cyp102A3. The cyp102A3 gene is preceded by, and organized in an operon with, a gene for a transcriptional regulator, encoded by fatR. The paralogous gene, cyp102A2, is most likely transcribed as a mono-cistronic message. We show that fatR encodes a protein that binds to an operator sequence that is present upstream of its own reading frame, thereby repressing the expression of the fatR-cyp102A3 operon. Unsaturated fatty acids and phytanic acid have the capacity to interact with FatR and to abrogate its binding to the operator sequence.

    Original languageEnglish
    Pages (from-to)459-464
    Number of pages6
    JournalArchives of Microbiology
    Volume176
    Issue number6
    DOIs
    Publication statusPublished - Dec 2001

    Keywords

    • Bacillus subtilis
    • Cyp102A1
    • Cyp102A2
    • Cyp102A3
    • Cytochrome P450
    • FatR
    • P450BM-3

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