Femtosecond optical injection of intact plant cells using a reconfigurable platform

Claire A. Mitchell; Stefan Kalies; Tomas Cizmar; Nicola Bellini; Anisha Kubasik-Thayil; Alexander Heisterkamp; Lesley Torrance; Alison G. Roberts; Frank J. Gunn-Moore; Kishan Dholakia

doi:10.1117/12.2037784

Femtosecond optical injection of intact plant cells using a reconfigurable platform

Claire A. Mitchell, Stefan Kalies, Tomas Cizmar, Nicola Bellini, Anisha Kubasik-Thayil, Alexander Heisterkamp, Lesley Torrance, Alison G. Roberts, Frank J. Gunn-Moore, Kishan Dholakia

Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

Original language	English
Title of host publication	Frontiers in Ultrafast Optics
Subtitle of host publication	Biomedical, Scientific, and Industrial Applications XIV
Editors	Alexander Heisterkamp, Peter R. Herman, Michel Meunier, Stefan Nolte
Place of Publication	Bellingham
Publisher	SPIE-International Society for Optical Engineering
ISBN (Print)	9780819498854
DOIs	https://doi.org/10.1117/12.2037784
Publication status	Published - 2014
Event	SPIE Photonics West 2014: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV - The Moscone Center, San Francisco, United States Duration: 2 Feb 2014 → 5 Feb 2014 http://spie.org/x106299.xml

Publication series

Name	Proceedings of SPIE
Publisher	SPIE
Volume	8972

Conference

Conference	SPIE Photonics West 2014: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV
Country/Territory	United States
City	San Francisco
Period	2/02/14 → 5/02/14
Internet address	http://spie.org/x106299.xml

Keywords

Ultrafast optics
Optoinjection
Photoporation
Plant cells
Bessel beams
Pulse dispersion compensation

Access to Document

10.1117/12.2037784

Cite this

Mitchell, C. A., Kalies, S., Cizmar, T., Bellini, N., Kubasik-Thayil, A., Heisterkamp, A., Torrance, L., Roberts, A. G., Gunn-Moore, F. J., & Dholakia, K. (2014). Femtosecond optical injection of intact plant cells using a reconfigurable platform. In A. Heisterkamp, P. R. Herman, M. Meunier, & S. Nolte (Eds.), Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV Article 89720C (Proceedings of SPIE; Vol. 8972). SPIE-International Society for Optical Engineering. https://doi.org/10.1117/12.2037784

Mitchell, Claire A. ; Kalies, Stefan ; Cizmar, Tomas et al. / Femtosecond optical injection of intact plant cells using a reconfigurable platform. Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV. editor / Alexander Heisterkamp ; Peter R. Herman ; Michel Meunier ; Stefan Nolte. Bellingham : SPIE-International Society for Optical Engineering, 2014. (Proceedings of SPIE).

@inproceedings{069d66849aaf41a2910db792fb7bb508,

title = "Femtosecond optical injection of intact plant cells using a reconfigurable platform",

abstract = "The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output. {\textcopyright} (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.",

keywords = "Ultrafast optics, Optoinjection, Photoporation, Plant cells, Bessel beams, Pulse dispersion compensation",

author = "Mitchell, {Claire A.} and Stefan Kalies and Tomas Cizmar and Nicola Bellini and Anisha Kubasik-Thayil and Alexander Heisterkamp and Lesley Torrance and Roberts, {Alison G.} and Gunn-Moore, {Frank J.} and Kishan Dholakia",

year = "2014",

doi = "10.1117/12.2037784",

language = "English",

isbn = "9780819498854",

series = "Proceedings of SPIE",

publisher = "SPIE-International Society for Optical Engineering",

editor = "Heisterkamp, {Alexander } and Herman, {Peter R. } and Meunier, {Michel } and Nolte, {Stefan }",

booktitle = "Frontiers in Ultrafast Optics",

note = "SPIE Photonics West 2014: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV ; Conference date: 02-02-2014 Through 05-02-2014",

url = "http://spie.org/x106299.xml",

}

Mitchell, CA, Kalies, S, Cizmar, T, Bellini, N, Kubasik-Thayil, A, Heisterkamp, A, Torrance, L, Roberts, AG, Gunn-Moore, FJ & Dholakia, K 2014, Femtosecond optical injection of intact plant cells using a reconfigurable platform. in A Heisterkamp, PR Herman, M Meunier & S Nolte (eds), Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV., 89720C, Proceedings of SPIE, vol. 8972, SPIE-International Society for Optical Engineering, Bellingham, SPIE Photonics West 2014: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV, San Francisco, United States, 2/02/14. https://doi.org/10.1117/12.2037784

Femtosecond optical injection of intact plant cells using a reconfigurable platform. / Mitchell, Claire A.; Kalies, Stefan; Cizmar, Tomas et al.
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV. ed. / Alexander Heisterkamp; Peter R. Herman; Michel Meunier; Stefan Nolte. Bellingham: SPIE-International Society for Optical Engineering, 2014. 89720C (Proceedings of SPIE; Vol. 8972).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Femtosecond optical injection of intact plant cells using a reconfigurable platform

AU - Mitchell, Claire A.

AU - Kalies, Stefan

AU - Cizmar, Tomas

AU - Bellini, Nicola

AU - Kubasik-Thayil, Anisha

AU - Heisterkamp, Alexander

AU - Torrance, Lesley

AU - Roberts, Alison G.

AU - Gunn-Moore, Frank J.

AU - Dholakia, Kishan

PY - 2014

Y1 - 2014

N2 - The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

AB - The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.

KW - Ultrafast optics

KW - Optoinjection

KW - Photoporation

KW - Plant cells

KW - Bessel beams

KW - Pulse dispersion compensation

U2 - 10.1117/12.2037784

DO - 10.1117/12.2037784

M3 - Conference contribution

SN - 9780819498854

T3 - Proceedings of SPIE

BT - Frontiers in Ultrafast Optics

A2 - Heisterkamp, Alexander

A2 - Herman, Peter R.

A2 - Meunier, Michel

A2 - Nolte, Stefan

PB - SPIE-International Society for Optical Engineering

CY - Bellingham

T2 - SPIE Photonics West 2014: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV

Y2 - 2 February 2014 through 5 February 2014

ER -

Mitchell CA, Kalies S, Cizmar T, Bellini N, Kubasik-Thayil A, Heisterkamp A et al. Femtosecond optical injection of intact plant cells using a reconfigurable platform. In Heisterkamp A, Herman PR, Meunier M, Nolte S, editors, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV. Bellingham: SPIE-International Society for Optical Engineering. 2014. 89720C. (Proceedings of SPIE). doi: 10.1117/12.2037784

Femtosecond optical injection of intact plant cells using a reconfigurable platform

Abstract

Publication series

Conference

Keywords

Access to Document

Fingerprint

Cite this