Abstract
The use of ultrashort-pulsed lasers for molecule delivery and transfection has proved to be a non-invasive and highly efficient technique for a wide range of mammalian cells. This present study investigates the effectiveness of femtosecond photoporation in plant cells, a hard-to-manipulate yet agriculturally relevant cell type, specifically suspension tobacco BY-2 cells. Both spatial and temporal shaping of the light field is employed to optimise the delivery of membrane impermeable molecules into plant cells using a reconfigurable optical system designed to be able to switch easily between different spatial modes and pulse durations. The use of a propagation invariant Bessel beam was found to increase the number of cells that could be viably optoinjected, when compared to the use of a Gaussian beam. Photoporation with a laser producing sub-12 fs pulses, coupled with a dispersion compensation system to retain the pulse duration at focus, reduced the power required for efficient optical injection by 1.5-1.8 times when compared to a photoporation with a 140 fs laser output. © (2014) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Original language | English |
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Title of host publication | Frontiers in Ultrafast Optics |
Subtitle of host publication | Biomedical, Scientific, and Industrial Applications XIV |
Editors | Alexander Heisterkamp, Peter R. Herman, Michel Meunier, Stefan Nolte |
Place of Publication | Bellingham |
Publisher | SPIE-International Society for Optical Engineering |
ISBN (Print) | 9780819498854 |
DOIs | |
Publication status | Published - 2014 |
Event | SPIE Photonics West 2014: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV - The Moscone Center, San Francisco, United States Duration: 2 Feb 2014 → 5 Feb 2014 http://spie.org/x106299.xml |
Publication series
Name | Proceedings of SPIE |
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Publisher | SPIE |
Volume | 8972 |
Conference
Conference | SPIE Photonics West 2014: Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XIV |
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Country/Territory | United States |
City | San Francisco |
Period | 2/02/14 → 5/02/14 |
Internet address |
Keywords
- Ultrafast optics
- Optoinjection
- Photoporation
- Plant cells
- Bessel beams
- Pulse dispersion compensation