TY - JOUR
T1 - Fertility testing and ICSI sperm selection by hyaluronic acid binding
T2 - Clinical and genetic aspects
AU - Huszar, Gabor
AU - Jakab, Attila
AU - Sakkas, Denny
AU - Ozenci, Ciler Celik
AU - Cayli, Sevil
AU - Delpiano, Elena
AU - Ozkavukcu, Sinan
N1 - Funding Information:
This research was supported by the NIH (HD-19505, HD-32902, OH-04061). The HA-related diagnostic and sperm selection devices were invented by GH, the invention was assigned to Yale University who owns the patent. Yale licensed the invention to Biocoat Inc. GH is acting as a scientific advisor.
PY - 2007/5
Y1 - 2007/5
N2 - The testis-expressed chaperone protein, HspA2 (previously creatine kinase M isoform) was established as a measure of human sperm cellular maturity, function and fertility. The presence of HspA2 in the synaptonemal complex is likely to link low HspA2 expression and increased frequency of chromosomal aneuploidies in arrested-maturity spermatozoa. A relationship also exists between HspA2 expression in elongating spermatids and the associated spermatogenetic events, including plasma membrane remodelling and the formation of zona pellucida and hyaluronic acid (HA) binding sites. The HA receptor of mature spermatozoa, when coupled with HA-coated slides and/or Petri dishes, allows visual observation of sperm-HA binding, providing a basis for sperm maturity testing, a major improvement in semen evaluation, and selection of mature spermatozoa for intracytoplasmic sperm injection (ICSI). Thus, in HA-selected spermatozoa the frequency of chromosomal disomy and diploidy is reduced 4- to 6-fold compared with semen sperm fractions. This reduction is similar to the increase in numerical chromosomal aberrations in ICSI children. Combined studies of sperm shape and chromosome probes demonstrated that sperm morphology does not aid selection of haploid spermatozoa. The HA-mediated sperm selection is a novel and efficient technique that may alleviate potential problems related to ICSI fertilization with visually selected spermatozoa.
AB - The testis-expressed chaperone protein, HspA2 (previously creatine kinase M isoform) was established as a measure of human sperm cellular maturity, function and fertility. The presence of HspA2 in the synaptonemal complex is likely to link low HspA2 expression and increased frequency of chromosomal aneuploidies in arrested-maturity spermatozoa. A relationship also exists between HspA2 expression in elongating spermatids and the associated spermatogenetic events, including plasma membrane remodelling and the formation of zona pellucida and hyaluronic acid (HA) binding sites. The HA receptor of mature spermatozoa, when coupled with HA-coated slides and/or Petri dishes, allows visual observation of sperm-HA binding, providing a basis for sperm maturity testing, a major improvement in semen evaluation, and selection of mature spermatozoa for intracytoplasmic sperm injection (ICSI). Thus, in HA-selected spermatozoa the frequency of chromosomal disomy and diploidy is reduced 4- to 6-fold compared with semen sperm fractions. This reduction is similar to the increase in numerical chromosomal aberrations in ICSI children. Combined studies of sperm shape and chromosome probes demonstrated that sperm morphology does not aid selection of haploid spermatozoa. The HA-mediated sperm selection is a novel and efficient technique that may alleviate potential problems related to ICSI fertilization with visually selected spermatozoa.
KW - Biochemical markers
KW - Chromosomal aneuploidies
KW - DNA fragmentation
KW - Hyaluronic acid binding
KW - ICSI
KW - Sperm maturity
UR - http://www.scopus.com/inward/record.url?scp=34248635671&partnerID=8YFLogxK
U2 - 10.1016/S1472-6483(10)61060-7
DO - 10.1016/S1472-6483(10)61060-7
M3 - Review article
C2 - 17509211
AN - SCOPUS:34248635671
SN - 1472-6483
VL - 14
SP - 650
EP - 663
JO - Reproductive BioMedicine Online
JF - Reproductive BioMedicine Online
IS - 5
M1 - 2697
ER -