FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability

Frederique M.D. Tholozan, Christopher Gribbon, Zheng Li, Martin W. Goldberg, Alan R. Prescott, Norman McKie, Roy A. Quinlan (Lead / Corresponding author)

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    51 Citations (Scopus)

    Abstract

    The lens is an avascular tissue, separated from the aqueous and vitreous humors by its own extracellular matrix, the lens capsule. Here we demonstrate that the lens capsule is a source of essential survival factors for lens epithelial cells. Primary and immortalized lens epithelial cells survive in low levels of serum and are resistant to staurosporine-induced apoptosis when they remain in contact with the lens capsule. Physical contact with the capsule is required for maximal resistance to stress. The lens capsule is also a source of soluble factors including fibroblast growth factor 2 (FGF-2) and perlecan, an extracellular matrix component that enhances FGF-2 activity. Matrix metalloproteinase 2 (MMP-2) inhibition as well as MMP-2 pretreatment of lens capsules greatly reduced the protective effect of the lens capsule, although this could be largely reversed by the addition of either conditioned medium or recombinant FGF-2. These data suggest that FGF-2 release from the lens capsule by MMP-2 is essential to lens epithelial cell viability and survival.

    Original languageEnglish
    Pages (from-to)4222-4231
    Number of pages10
    JournalMolecular Biology of the Cell
    Volume18
    Issue number11
    DOIs
    Publication statusPublished - 1 Nov 2007

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    Tholozan, F. M. D., Gribbon, C., Li, Z., Goldberg, M. W., Prescott, A. R., McKie, N., & Quinlan, R. A. (2007). FGF-2 Release from the Lens Capsule by MMP-2 Maintains Lens Epithelial Cell Viability. Molecular Biology of the Cell, 18(11), 4222-4231. https://doi.org/10.1091/mbc.E06-05-0416