FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk

Patricia L. Martinez-Morales, Ruth Diez del Corral, Isabel Olivera-Martinez, Alejandra C. Quiroga, Raman M. Das, Julio A. Barbas, Kate G. Storey, Aixa V. Morales

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    Abstract

    Coordination between functionally related adjacent tissues is essential during development. For example, formation of trunk neural crest cells (NCCs) is highly influenced by the adjacent mesoderm, but the molecular mechanism involved is not well understood. As part of this mechanism, fibroblast growth factor (FGF) and retinoic acid (RA) mesodermal gradients control the onset of neurogenesis in the extending neural tube. In this paper, using gain-and loss-of-function experiments, we show that caudal FGF signaling prevents premature specification of NCCs and, consequently, premature epithelial-mesenchymal transition (EMT) to allow cell emigration. In contrast, rostrally generated RA promotes EMT of NCCs at somitic levels. Furthermore, we show that FGF and RA signaling control EMT in part through the modulation of elements of the bone morphogenetic protein and Wnt signaling pathways. These data establish a clear role for opposition of FGF and RA signaling in control of the timing of NCC EMT and emigration and, consequently, co-ordination of the development of the central and peripheral nervous system during vertebrate trunk elongation.

    Original languageEnglish
    Pages (from-to)489-503
    Number of pages15
    JournalJournal of Cell Biology
    Volume194
    Issue number3
    DOIs
    Publication statusPublished - 1 Aug 2011

    Keywords

    • Epithelial mesenchymal transition
    • Transcription factor FoxD3
    • Tyrosine kinase domain
    • Vertebrate body axis
    • Neuronal differentiation
    • Signaling controls
    • Paraxial mesoderm
    • Fate restrictions
    • Opposing FGF
    • Chick embryo

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