FGF signalling regulates chromatin organisation during neural differentiation via mechanisms that can be uncoupled from transcription

Nishal S. Patel, Muriel Rhinn, Claudia I. Semprich, Pamela A. Halley, Pascal Dollé, Wendy A. Bickmore (Lead / Corresponding author), Kate G. Storey (Lead / Corresponding author)

    Research output: Contribution to journalArticle

    31 Citations (Scopus)

    Abstract

    Changes in higher order chromatin organisation have been linked to transcriptional regulation; however, little is known about how such organisation alters during embryonic development or how it is regulated by extrinsic signals. Here we analyse changes in chromatin organisation as neural differentiation progresses, exploiting the clear spatial separation of the temporal events of differentiation along the elongating body axis of the mouse embryo. Combining fluorescence in situ hybridisation with super-resolution structured illumination microscopy, we show that chromatin around key differentiation gene loci Pax6 and Irx3 undergoes both decompaction and displacement towards the nuclear centre coincident with transcriptional onset. Conversely, down-regulation of Fgf8 as neural differentiation commences correlates with a more peripheral nuclear position of this locus. During normal neural differentiation, fibroblast growth factor (FGF) signalling is repressed by retinoic acid, and this vitamin A derivative is further required for transcription of neural genes. We show here that exposure to retinoic acid or inhibition of FGF signalling promotes precocious decompaction and central nuclear positioning of differentiation gene loci. Using the Raldh2 mutant as a model for retinoid deficiency, we further find that such changes in higher order chromatin organisation are dependent on retinoid signalling. In this retinoid deficient condition, FGF signalling persists ectopically in the elongating body, and importantly, we find that inhibiting FGF receptor (FGFR) signalling in Raldh2-/- embryos does not rescue differentiation gene transcription, but does elicit both chromatin decompaction and nuclear position change. These findings demonstrate that regulation of higher order chromatin organisation during differentiation in the embryo can be uncoupled from the machinery that promotes transcription and, for the first time, identify FGF as an extrinsic signal that can direct chromatin compaction and nuclear organisation of gene loci.
    Original languageEnglish
    Article numbere1003614
    JournalPLoS Genetics
    Volume9
    Issue number7
    DOIs
    Publication statusPublished - 18 Jul 2013

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    fibroblast growth factors
    Fibroblast Growth Factors
    Chromatin
    chromatin
    transcription (genetics)
    retinoids
    Retinoids
    gene
    embryo (animal)
    loci
    Embryonic Structures
    Genes
    retinoic acid
    embryo
    Tretinoin
    genes
    Fibroblast Growth Factor Receptors
    fluorescence in situ hybridization
    Lighting
    Vitamin A

    Cite this

    Patel, Nishal S. ; Rhinn, Muriel ; Semprich, Claudia I. ; Halley, Pamela A. ; Dollé, Pascal ; Bickmore, Wendy A. ; Storey, Kate G. / FGF signalling regulates chromatin organisation during neural differentiation via mechanisms that can be uncoupled from transcription. In: PLoS Genetics. 2013 ; Vol. 9, No. 7.
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    abstract = "Changes in higher order chromatin organisation have been linked to transcriptional regulation; however, little is known about how such organisation alters during embryonic development or how it is regulated by extrinsic signals. Here we analyse changes in chromatin organisation as neural differentiation progresses, exploiting the clear spatial separation of the temporal events of differentiation along the elongating body axis of the mouse embryo. Combining fluorescence in situ hybridisation with super-resolution structured illumination microscopy, we show that chromatin around key differentiation gene loci Pax6 and Irx3 undergoes both decompaction and displacement towards the nuclear centre coincident with transcriptional onset. Conversely, down-regulation of Fgf8 as neural differentiation commences correlates with a more peripheral nuclear position of this locus. During normal neural differentiation, fibroblast growth factor (FGF) signalling is repressed by retinoic acid, and this vitamin A derivative is further required for transcription of neural genes. We show here that exposure to retinoic acid or inhibition of FGF signalling promotes precocious decompaction and central nuclear positioning of differentiation gene loci. Using the Raldh2 mutant as a model for retinoid deficiency, we further find that such changes in higher order chromatin organisation are dependent on retinoid signalling. In this retinoid deficient condition, FGF signalling persists ectopically in the elongating body, and importantly, we find that inhibiting FGF receptor (FGFR) signalling in Raldh2-/- embryos does not rescue differentiation gene transcription, but does elicit both chromatin decompaction and nuclear position change. These findings demonstrate that regulation of higher order chromatin organisation during differentiation in the embryo can be uncoupled from the machinery that promotes transcription and, for the first time, identify FGF as an extrinsic signal that can direct chromatin compaction and nuclear organisation of gene loci.",
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    FGF signalling regulates chromatin organisation during neural differentiation via mechanisms that can be uncoupled from transcription. / Patel, Nishal S.; Rhinn, Muriel; Semprich, Claudia I.; Halley, Pamela A.; Dollé, Pascal; Bickmore, Wendy A. (Lead / Corresponding author); Storey, Kate G. (Lead / Corresponding author).

    In: PLoS Genetics, Vol. 9, No. 7, e1003614, 18.07.2013.

    Research output: Contribution to journalArticle

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    AU - Patel, Nishal S.

    AU - Rhinn, Muriel

    AU - Semprich, Claudia I.

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