Fibre optic time-resolved spectroscopy using CMOS-SPAD arrays

K. Ehrlich, A. Kufcsák, N. Krstajić, R. K. Henderson, R. R. Thomson, M. G. Tanner

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    7 Citations (Scopus)

    Abstract

    In vivo fibre optic fluorescence-based sensing is the use of synthesised fluorophores which interrogate the local environment via variation in their fluorescence emission, addressed through an optic fibre. However, the emission intensity is influenced by intrinsic factors such as photobleaching, quantitative factors like concentration dependency and background signals from autofluorescence of tissue and the delivery optical fibre. Many of these problems can be addressed by using time-resolved spectroscopy which measures variations in the fluorescent lifetime. We present a versatile fibre-based time-resolved spectrograph based on a CMOS SPAD line sensor capable of acquiring time and spectral resolved fluorescent lifetime data in a single measurement exploiting the time-correlated single photon counting (TCSPC) technique. It is shown that these TCSPC histograms enable the differentiation between autofluorescence of tissue and synthesized fluorophores, as well as the removal of unwanted fibre background through post-processed time-gating. As a proof-of-principle application the pH- dependent changes in fluorescent lifetime of 5-carboxyuorescein (FAM) are measured.

    Original languageEnglish
    Title of host publicationOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII
    EditorsIsrael Gannot
    PublisherSPIE-International Society for Optical Engineering
    Number of pages8
    Volume10058
    ISBN (Electronic)9781510605572
    DOIs
    Publication statusPublished - 28 Feb 2017
    EventOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII - San Francisco, United States
    Duration: 28 Jan 201729 Jan 2017

    Conference

    ConferenceOptical Fibers and Sensors for Medical Diagnostics and Treatment Applications XVII
    CountryUnited States
    CitySan Francisco
    Period28/01/1729/01/17

    Keywords

    • CMOS line arrays
    • Laser-induced uorescence
    • Lifetime-based sensing
    • TCSPC
    • Time-resolved spectroscopy

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