Abstract
Fibroblast growth factor (FGF)-dependent epithelial-mesenchymal transitions and cell migration contribute to the establishment of germ layers in vertebrates and other animals, but a comprehensive demonstration of the cellular activities that FGF controls to mediate these events has not been provided for any system. The establishment of the Drosophila mesoderm layer from an epithelial primordium involves a transition to a mesenchymal state and the dispersal of cells away from the site of internalisation in a FGF-dependent fashion. We show here that FGF plays multiple roles at successive stages of mesoderm morphogenesis in Drosophila. It is first required for the mesoderm primordium to lose its epithelial polarity. An intimate, FGF-dependent contact is established and maintained between the germ layers through mesoderm cell protrusions. These protrusions extend deep into the underlying ectoderm epithelium and are associated with high levels of E-cadherin at the germ layer interface. Finally, FGF directs distinct hitherto unrecognised and partially redundant protrusive behaviours during later mesoderm spreading. Cells first move radially towards the ectoderm, and then switch to a dorsally directed movement across its surface. We show that both movements are important for layer formation and present evidence suggesting that they are controlled by genetically distinct mechanisms.
| Original language | English |
|---|---|
| Pages (from-to) | 2705-2715 |
| Number of pages | 11 |
| Journal | Development |
| Volume | 138 |
| Issue number | 13 |
| Early online date | 25 May 2011 |
| DOIs | |
| Publication status | Published - 1 Jul 2011 |
Keywords
- Cell signalling
- Gastrulation
- Cell migration
- E-cadherin
- Fibroblast growth factor
- Drosophila
- Adherens junctions
- Primitive streak
- Larval cuticle
- Shape changes
- Zygotic loci
- DE-cadherin
- Migration
- Gastrulation
- Embryos
- Melanogaster
Cite this
}
Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila. / Clark, Ivan B. N.; Muha, Villo; Klingseisen, Anna; Leptin, Maria; Muller, Hans-Arno J. (Lead / Corresponding author).
In: Development, Vol. 138, No. 13, 01.07.2011, p. 2705-2715.Research output: Contribution to journal › Article
TY - JOUR
T1 - Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila
AU - Clark, Ivan B. N.
AU - Muha, Villo
AU - Klingseisen, Anna
AU - Leptin, Maria
AU - Muller, Hans-Arno J.
PY - 2011/7/1
Y1 - 2011/7/1
N2 - Fibroblast growth factor (FGF)-dependent epithelial-mesenchymal transitions and cell migration contribute to the establishment of germ layers in vertebrates and other animals, but a comprehensive demonstration of the cellular activities that FGF controls to mediate these events has not been provided for any system. The establishment of the Drosophila mesoderm layer from an epithelial primordium involves a transition to a mesenchymal state and the dispersal of cells away from the site of internalisation in a FGF-dependent fashion. We show here that FGF plays multiple roles at successive stages of mesoderm morphogenesis in Drosophila. It is first required for the mesoderm primordium to lose its epithelial polarity. An intimate, FGF-dependent contact is established and maintained between the germ layers through mesoderm cell protrusions. These protrusions extend deep into the underlying ectoderm epithelium and are associated with high levels of E-cadherin at the germ layer interface. Finally, FGF directs distinct hitherto unrecognised and partially redundant protrusive behaviours during later mesoderm spreading. Cells first move radially towards the ectoderm, and then switch to a dorsally directed movement across its surface. We show that both movements are important for layer formation and present evidence suggesting that they are controlled by genetically distinct mechanisms.
AB - Fibroblast growth factor (FGF)-dependent epithelial-mesenchymal transitions and cell migration contribute to the establishment of germ layers in vertebrates and other animals, but a comprehensive demonstration of the cellular activities that FGF controls to mediate these events has not been provided for any system. The establishment of the Drosophila mesoderm layer from an epithelial primordium involves a transition to a mesenchymal state and the dispersal of cells away from the site of internalisation in a FGF-dependent fashion. We show here that FGF plays multiple roles at successive stages of mesoderm morphogenesis in Drosophila. It is first required for the mesoderm primordium to lose its epithelial polarity. An intimate, FGF-dependent contact is established and maintained between the germ layers through mesoderm cell protrusions. These protrusions extend deep into the underlying ectoderm epithelium and are associated with high levels of E-cadherin at the germ layer interface. Finally, FGF directs distinct hitherto unrecognised and partially redundant protrusive behaviours during later mesoderm spreading. Cells first move radially towards the ectoderm, and then switch to a dorsally directed movement across its surface. We show that both movements are important for layer formation and present evidence suggesting that they are controlled by genetically distinct mechanisms.
KW - Cell signalling
KW - Gastrulation
KW - Cell migration
KW - E-cadherin
KW - Fibroblast growth factor
KW - Drosophila
KW - Adherens junctions
KW - Primitive streak
KW - Larval cuticle
KW - Shape changes
KW - Zygotic loci
KW - DE-cadherin
KW - Migration
KW - Gastrulation
KW - Embryos
KW - Melanogaster
UR - http://www.scopus.com/inward/record.url?scp=79958086467&partnerID=8YFLogxK
U2 - 10.1242/dev.060277
DO - 10.1242/dev.060277
M3 - Article
VL - 138
SP - 2705
EP - 2715
JO - Development
JF - Development
SN - 0950-1991
IS - 13
ER -