FlgN is required for flagellum based motility by Bacillus subtilis

Lynne S. Cairns, Victoria L. Marlow, Taryn B. Kiley, Christopher Birchall, Adam Ostrowski, Phillip D. Aldridge, Nicola R. Stanley-Wall (Lead / Corresponding author)

    Research output: Contribution to journalArticle

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    153 Downloads (Pure)

    Abstract

    The assembly of the bacterial flagellum is exquisitely controlled. Flagellar biosynthesis is underpinned by a specialized type III secretion system that allows export of proteins from the cytoplasm to the nascent structure. Bacillus subtilisregulates flagellar assembly using both conserved and species-specific mechanisms. Here, we show that YvyG is essential for flagellar filament assembly. We define YvyG as an orthologue of the Salmonella enterica serovar Typhimurium type III secretion system chaperone, FlgN, which is required for the export of the hook-filament junction proteins, FlgK and FlgL. Deletion of flgN (yvyG) results in a nonmotile phenotype that is attributable to a decrease in hag translation and a complete lack of filament polymerization. Analyses indicate that a flgK-flgL double mutant strain phenocopies deletion of flgN and that overexpression of flgK-flgLcannot complement the motility defect of a ΔflgN strain. Furthermore, in contrast to previous work suggesting that phosphorylation of FlgN alters its subcellular localization, we show that mutation of the identified tyrosine and arginine FlgN phosphorylation sites has no effect on motility. These data emphasize that flagellar biosynthesis is differentially regulated in B. subtilis from classically studied Gram-negative flagellar systems and questions the biological relevance of some posttranslational modifications identified by global proteomic approaches.


    Original languageEnglish
    Pages (from-to)2216-2226
    Number of pages11
    JournalJournal of Bacteriology
    Volume196
    Issue number12
    Early online date4 Apr 2014
    DOIs
    Publication statusPublished - 2014

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  • Student Theses

    Probing the assembly of the Bacillus subtilis flagellum and its role in signal transduction.

    Author: Cairns, L. S., 2014

    Supervisor: Stanley-Wall, N. (Supervisor)

    Student thesis: Doctoral ThesisDoctor of Philosophy

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    Cite this

    Cairns, L. S., Marlow, V. L., Kiley, T. B., Birchall, C., Ostrowski, A., Aldridge, P. D., & Stanley-Wall, N. R. (2014). FlgN is required for flagellum based motility by Bacillus subtilis. Journal of Bacteriology, 196(12), 2216-2226. https://doi.org/10.1128/JB.01599-14