Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Anna Chiara De Luca, Michael Mazilu, Andrew Riches, Simon Herrington, Kishan Dholakia

    Research output: Chapter in Book/Report/Conference proceedingConference contribution


    The use of Raman spectroscopy for biomedical applications requires overcoming the obstacle of the broad fluorescence background that is generally generated in biological samples. Recently, we have developed a new modulation method for separating the weak Raman peaks from the strong fluorescence background. The novel method is based on the periodical modulation of the excitation wavelength and uses the principle of multi-channel lock-in detection. By continuously modulating the excitation wavelength it is possible to shift the Raman peaks while the fluorescence background remains essentially constant. The powerful capabilities of this novel method are demonstrated by acquiring spectra from different location (nucleus, cytoplasm and membrane) inside a CHO cell. In fact, we show that our modulated Raman spectroscopy provides, with higher efficiency than the standard one, Raman spectra of different locations within a single cell, suggesting that this minimally invasive optical technology could be applied for bio-medical diagnosis and imaging.

    Original languageEnglish
    Title of host publicationAdvanced Biomedical and Clinical Diagnostic Systems VIII
    EditorsTuan Vo-Dinh, Warren S. Grundfest, Anita Mahadevan-Jansen
    Place of PublicationBellingham
    PublisherSPIE-International Society for Optical Engineering
    ISBN (Print)9780819479518
    Publication statusPublished - 2010
    EventSPIE Photonics West 2010: Advanced Biomedical and Clinical Diagnostic Systems VIII - The Moscone Center, San Francisco, United States
    Duration: 24 Jan 201026 Jan 2010

    Publication series

    NameProceedings of SPIE


    ConferenceSPIE Photonics West 2010: Advanced Biomedical and Clinical Diagnostic Systems VIII
    Country/TerritoryUnited States
    CitySan Francisco
    Internet address


    • Raman spectroscopy
    • fluorescence-free
    • PCA


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