Fluorescence lifetime imaging

Klaus Suhling, Liisa M. Hirvonen, James A. Levitt, Pei Hua Chung, Carolyn Tregidgo, Dmitri A. Rusakov, Kaiyu Zheng, Simon Ameer-Beg, Simon P. Poland, Simao Coelho, Robert Henderson, Nikola Krstajic

    Research output: Chapter in Book/Report/Conference proceedingChapter

    6 Citations (Scopus)


    Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photophysical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is largely independent of the local fluorophore concentration and excitation intensity. Many FLIM applications relevant for biology concern the identification of Förster resonance energy transfer (FRET) to study protein interactions and conformational changes. In addition, FLIM has been used to image viscosity, temperature, pH, refractive index, and ion and oxygen concentrations, all at the cellular level. The basic principles and recent advances in the application of FLIM, FLIM instrumentation, molecular probe, and FLIM detector development will be discussed.

    Original languageEnglish
    Title of host publicationHandbook of Photonics for Biomedical Engineering
    EditorsAaron Ho-Pui Ho, Donghyun Kim, Michael G. Somekh
    Place of PublicationDordrecht
    Number of pages53
    ISBN (Electronic)9789400750524
    ISBN (Print)9789400750517
    Publication statusPublished - 2017


    • Anisotropy
    • Fluorescence anisotropy imaging (FAIM)
    • Fluorescence enhancement
    • Fluorescence microscopy
    • Fluorescence spectroscopy
    • Förster resonance energy transfer (FRET)
    • Plasmonics
    • Time-correlated single-photon counting (TCSPC)
    • Time-resolved fluorescence anisotropy imaging (TR-FAIM)
    • Total internal reflection fluorescence (TIRF)

    ASJC Scopus subject areas

    • General Biochemistry,Genetics and Molecular Biology
    • General Engineering
    • General Physics and Astronomy


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