Projects per year
Synthetic hexynyl α-D-mannopyranoside and its α-1,6-linked disaccharide counterpart were fluorescently labelled through CuAAC click chemistry with 3-azido-7-hydroxycoumarin. The resulting triazolyl-coumarin adducts, which were amenable to analysis by TLC, HPLC and mass spectrometry, proved to be acceptor substrates for α-1,6-ManT activities in mycobacterial membranes, as well as α- and β-GalT activities in trypanosomal membranes, benchmarking the potential of the fluorescent acceptor approach against earlier radiochemical assays. Following on to explore the glycobiology of the benign protozoan alga Euglena gracilis, α-1,3- and α-1,2-ManT activities were detected in membrane preparations, along with GlcT, Glc-P-T and GlcNAc-P-T activities. These studies serve to demonstrate the potential of readily accessible fluorescent glycans as substrates for exploring carbohydrate active enzymes.
|Number of pages||13|
|Early online date||30 Nov 2016|
|Publication status||Published - 13 Jan 2017|
- Euglena gracilis
- Fluorescent glycans
- Enzyme assays
FingerprintDive into the research topics of 'Fluorescent mannosides serve as acceptor substrates for glycosyltransferase and sugar-1-phosphate transferase activities in Euglena gracilis membranes'. Together they form a unique fingerprint.
- 1 Active
Protein Glycosylation in Trypanosomes: Defining and Exploiting a Biological System (Senior Investigator Award)
1/10/13 → 30/06/23