Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

Hong Yin; Cecil Chan; Jacqui Garforth; Kenneth T. Douglas; Mark S. Bolgar; Simon J. Gaskell; Alan H. Fairlamb

doi:10.1039/cc9960000973

Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

Hong Yin, Cecil Chan, Jacqui Garforth, Kenneth T. Douglas, Mark S. Bolgar, Simon J. Gaskell, Alan H. Fairlamb

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.

Original language	English
Pages (from-to)	973-974
Number of pages	2
Journal	Chemical Communications
Issue number	8
DOIs	https://doi.org/10.1039/cc9960000973
Publication status	Published - 1996

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/cc9960000973

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title = "Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase",

abstract = "Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.",

author = "Hong Yin and Cecil Chan and Jacqui Garforth and Douglas, {Kenneth T.} and Bolgar, {Mark S.} and Gaskell, {Simon J.} and Fairlamb, {Alan H.}",

year = "1996",

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language = "English",

pages = "973--974",

journal = "Chemical Communications",

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T1 - Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

AU - Yin, Hong

AU - Chan, Cecil

AU - Garforth, Jacqui

AU - Douglas, Kenneth T.

AU - Bolgar, Mark S.

AU - Gaskell, Simon J.

AU - Fairlamb, Alan H.

PY - 1996

Y1 - 1996

N2 - Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.

AB - Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.

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U2 - 10.1039/cc9960000973

DO - 10.1039/cc9960000973

M3 - Article

AN - SCOPUS:0029874658

SN - 1359-7345

SP - 973

EP - 974

JO - Chemical Communications

JF - Chemical Communications

IS - 8

ER -

Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

Abstract

ASJC Scopus subject areas

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