Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

Hong Yin; Cecil Chan; Jacqui Garforth; Kenneth T. Douglas; Mark S. Bolgar; Simon J. Gaskell; Alan H. Fairlamb

doi:10.1039/cc9960000973

Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

Hong Yin
, Cecil Chan
, Jacqui Garforth
, Kenneth T. Douglas
, Mark S. Bolgar
, Simon J. Gaskell
, Alan H. Fairlamb

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.

Original language	English
Pages (from-to)	973-974
Number of pages	2
Journal	Chemical Communications
Issue number	8
DOIs	https://doi.org/10.1039/cc9960000973
Publication status	Published - 1996

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
General Chemistry
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/cc9960000973

Cite this

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title = "Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase",

abstract = "Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.",

author = "Hong Yin and Cecil Chan and Jacqui Garforth and Douglas, \{Kenneth T.\} and Bolgar, \{Mark S.\} and Gaskell, \{Simon J.\} and Fairlamb, \{Alan H.\}",

year = "1996",

doi = "10.1039/cc9960000973",

language = "English",

pages = "973--974",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

number = "8",

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T1 - Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

AU - Yin, Hong

AU - Chan, Cecil

AU - Garforth, Jacqui

AU - Douglas, Kenneth T.

AU - Bolgar, Mark S.

AU - Gaskell, Simon J.

AU - Fairlamb, Alan H.

PY - 1996

Y1 - 1996

N2 - Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.

AB - Photolysis of fluphenazine, a competitive inhibitor of trypanothione reductase in the presence of trypanothione reductase leads to irreversible, time-dependent inactivation, which is not dependent on the presence of molecular oxygen in the medium and can be protected against by the presence of trypanothione substrate; MALDI and electrospray mass spectrometric analyses shows that 2-5 equiv. of the phenothiazine are incorporated per enzyme subunit.

UR - https://www.scopus.com/pages/publications/0029874658

U2 - 10.1039/cc9960000973

DO - 10.1039/cc9960000973

M3 - Article

AN - SCOPUS:0029874658

SN - 1359-7345

SP - 973

EP - 974

JO - Chemical Communications

JF - Chemical Communications

IS - 8

ER -

Fluphenazine photoaffinity labelling of binding sites for phenothiazine inhibitors of trypanothione reductase

Abstract

ASJC Scopus subject areas

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