FORGETTER2 protein phosphatase and phospholipase D modulate heat stress memory in Arabidopsis

TY - JOUR

T1 - FORGETTER2 protein phosphatase and phospholipase D modulate heat stress memory in Arabidopsis

AU - Urrea Castellanos, Reynel

AU - Friedrich, Thomas

AU - Petrovic, Nevena

AU - Altmann, Simone

AU - Brzezinka, Krzysztof

AU - Gorka, Michal

AU - Graf, Alexander

AU - Bäurle, Isabel

N1 - Funding Information: We acknowledge NASC and RIKEN for seeds, C. Kappel for bioinformatics support, F. J?hne, S. Wenk, and E. Benke for technical assistance, and C. Schmidt and D. M?ker for excellent plant care. We also thank M. Lenhard, A. Sicard, and members of our laboratory for helpful comments. IB acknowledges support from an ERC CoG (725295), a Sofja-Kovalevskaja-Award (Alexander-von-Humboldt-Foundation), and CRC 973 Project A2 (DFG). RUC was supported by scholarships from the CRC 973, the Potsdam Graduate School, and the IMPRS-PMPG program of the Max-Planck-Society. Open access funding enabled and organized by ProjektDEAL. Publisher Copyright: © 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/9/1

Y1 - 2020/9/1

N2 - Plants can mitigate environmental stress conditions through acclimation. In the case of fluctuating stress conditions such as high temperatures, maintaining a stress memory enables a more efficient response upon recurring stress. In a genetic screen for Arabidopsis thaliana mutants impaired in the memory of heat stress (HS) we have isolated the FORGETTER2 (FGT2) gene, which encodes a type 2C protein phosphatase (PP2C) of the D-clade. Fgt2 mutants acquire thermotolerance normally; however, they are defective in the memory of HS. FGT2 interacts with phospholipase D α2 (PLDα2), which is involved in the metabolism of membrane phospholipids and is also required for HS memory. In summary, we have uncovered a previously unknown component of HS memory and identified the FGT2 protein phosphatase and PLDα2 as crucial players, suggesting that phosphatidic acid-dependent signaling or membrane composition dynamics underlie HS memory.

AB - Plants can mitigate environmental stress conditions through acclimation. In the case of fluctuating stress conditions such as high temperatures, maintaining a stress memory enables a more efficient response upon recurring stress. In a genetic screen for Arabidopsis thaliana mutants impaired in the memory of heat stress (HS) we have isolated the FORGETTER2 (FGT2) gene, which encodes a type 2C protein phosphatase (PP2C) of the D-clade. Fgt2 mutants acquire thermotolerance normally; however, they are defective in the memory of HS. FGT2 interacts with phospholipase D α2 (PLDα2), which is involved in the metabolism of membrane phospholipids and is also required for HS memory. In summary, we have uncovered a previously unknown component of HS memory and identified the FGT2 protein phosphatase and PLDα2 as crucial players, suggesting that phosphatidic acid-dependent signaling or membrane composition dynamics underlie HS memory.

KW - priming

KW - protein phosphatase

KW - stress memory

KW - heat stress

KW - Arabidopsis thaliana

UR - https://www.scopus.com/pages/publications/85089024674

U2 - 10.1111/tpj.14927

DO - 10.1111/tpj.14927

M3 - Article

C2 - 32654320

SN - 0960-7412

VL - 104

SP - 7

EP - 17

JO - Plant Journal

JF - Plant Journal

IS - 1

ER -